Mouse Adiponectin Receptor 1 (ADIPOR1) ELISA Kit

SKU: orb776708

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Adiponectin Receptor 1(ADIPOR1).

Images & Validation

• Tested Applications: ELISA
• Application Notes:
  standard: 200 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ADIPOR1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ADIPOR1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ADIPOR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ADIPOR1 in the samples is then determined by comparing the OD of the samples to the standard curve

Key Properties

• Reactivity: Mouse
• Sample Types: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Assay Time: 3.5h
• Range: 3.13-200 ng/mL
• Sensitivity: 1.19 ng/mL
• Concentration: 200 ng/mL

Storage & Handling

• Storage: Refer to the Storage Guidelines in the Manual
• Disclaimer: For research use only

Alternative Names

ACDCR1 ELISA Kit, CGI45 ELISA Kit, PAQR1 ELISA Kit, TESBP1A ELISA Kit, TESBP1-A ELISA Kit, Progestin and AdipoQ Receptor Family Member I ELISA Kit

Quick Database Links

UniProt

Q91VH1