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Mouse Acetylcholinesterase (AChE) ELISA Kit

Catalog Number: orb1808031

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb1808031
CategoryAssays and Kits
DescriptionMouse Acetylcholinesterase (AChE) ELISA Kit
TargetAChE
ReactivityMouse
UniProt IDP21836
Application notesThis ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse AChE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse AChE. You can calculate the concentration of Mouse AChE in the samples by comparing the OD of the samples to the standard curve.
Assay TypeSandwich
Assay Time3.5H
Standard20ng/mL
Range0.31-20ng/mL
Sensitivity0.19 ng/mL
Sample Typesserum, plasma, Tissue homogenate and Other biological samples
Alternative namesACEE, ARACHE, N-ACHE, YT, acetylhydrolase
NoteFor research use only
Mouse Acetylcholinesterase (AChE) ELISA Kit

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