Mouse 17-Beta-Hydroxysteroid Dehydrogenase Type 10 (HSD17b10) ELISA Kit

• Catalog Number: orb780816
• Category: Assays and Kits
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to 17-Beta-Hydroxysteroid Dehydrogenase Type 10(HSD17b10).
• Reactivity: Mouse
• Concentration: 10 ng/mL
• UniProt ID: O08756
• Tested applications: ELISA
• Application notes: standard: 10 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HSD17b10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HSD17b10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HSD17b10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HSD17b10 in the samples is then determined by comparing the OD of the samples to the standard curve
• Assay Time: 3.5h
• Range: 0.16-10 ng/mL
• Sensitivity: 0.061 ng/mL
• Sample Types: Tissue homogenates and other biological fluids.
• Alternative names: 17b-HSD10 ELISA Kit, ABAD ELISA Kit, ERAB ELISA Ki
• Note: For research use only