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Description
Research Area
Images & Validation
−| Tested Applications | WB |
|---|---|
| Application Notes |
Key Properties
−| Source | HEK293 cells |
|---|---|
| Biological Activity | Measured by its ability to cleave the fluorogenic peptide substrate, Mca-PLGL-Dpa-AR-NH2. The specific activity is >1200 pmol/min/μg. |
| Target | MMP9 |
| Tag | His Tag |
| MW | 50.8 kDa |
| Purity | >95% as determined by SDS-PAGE. |
| Protein Sequence | Ala 20 - Pro 469 |
Storage & Handling
−| Storage | Lyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 |
| Expiration Date | 6 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−Human MMP9(20-469) Protein, His Tag [orb1290955]
Unconjugated
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 48.5 kDa after removal of the signal peptide. The apparent molecular mass of MMP9-His is approximately 55-70 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μgMMP9 Rabbit Polyclonal Antibody [orb1289729]
IF, IHC, WB
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl, 30 μlHuman MMP9(20-707) Protein, His Tag [orb1290924]
Unconjugated
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
The protein has a predicted molecular mass of 77.2 kDa after removal of the signal peptide. The apparent molecular mass of MMP9(20-707)-His is approximately 70-100 kDa due to glycosylation.
Mammalian
10 μg, 100 μg, 50 μg

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Human MMP-9, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
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Protocol Information
MMP9 Recombinant Protein (orb1227954)
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