MMAF

SKU: orb1304379

Description

MonoMethyl auristatin F (MMAF) is a potent antimitotic agent that disrupts microtubule dynamics by inhibiting tubulin polymerization, thereby arresting cell division. This mechanism makes it a valuable payload in antibody-drug conjugates (ADCs) for cancer research, with applications in both in vitro cytotoxicity assays and in vivo xenograft studies.

Research Area

Signal Transduction

Key Properties

• CAS Number: 745017-94-1
• MW: 731.962
• Purity: 99.88% (May vary between batches)
• Formula: C₃₉H₆₅N₅O₈
• SMILES: O=C(O)[C@@H](NC([C@H](C)[C@H]([C@H]1N(C(C[C@@H](OC)[C@@H](N(C)C([C@@H](NC([C@@H](NC)C(C)C)=O)C(C)C)=O)[C@@H](C)CC)=O)CCC1)OC)=O)CC2=CC=CC=C2
• Target: ADC Cytotoxin,Microtubule Associated
• Solubility: DMSO:250 mg/mL (341.55 mM)

Bioactivity

• Target IC50:
  H3397 cells:105 nM.
• In Vivo:
  The maximum tolerated dose in mice of MMAF (>16 mg/kg) is much higher than MMAE (1 mg/kg). cAC10-L1-MMAF4 has an MTD of 50 mg/kg in mice and 15 mg/kg in rats. The corresponding cAC10-L4-MMAF4 ADC was much less toxic, having MTDs in mice and rats of >150 mg/ kg and 90 mg/kg in rats, respectively.
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• In Vitro:
  MMAF shows in vitro cytotoxicity against a panel of cell lines. The IC50 values for Karpas 299, H3396, 786-O and Caki-1 are 119, 105, 257, and 200 nM, respectively. Targeted MMAF is much more potent than the free drug, and that cAC10 conjugates of MMAF display pronounced activities. On a molar basis, the cAC10-L1-MMAF4 is an average of over 2200-fold more potent than free MMAF and is active on all the CD30-positive cell lines tested.
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• Cell Research:
  Cells are treated with serial dilutions of test molecules and incubated 4-6 days depending on cell line. Assessment of cellular growth and data reduction to generate IC50 values is done using Alamar Blue dye reduction assay.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ADC Payload, ADCCytotoxin, ADC Cytotoxin, Cytotoxicity( Karpas 299 cell), Inhibitor, MonoMethyl auristatin F, Monomethylauristatin F, MMAF, Microtubule Associated, Microtubule/Tubulin, MicrotubuleAssociated, inhibit

Compound Identifiers

PubChem CID

56841603