ML216

SKU: orb1301810

Description

ML216 (CID-49852229) is a potent and selective inhibitor of the BLM helicase with an IC50 of 1.8 µM, demonstrating over 28-fold selectivity against RECQ1, RECQ5, and E. coli UvrD. This tool compound is used in vitro to study DNA repair mechanisms, replication stress, and genomic instability associated with Bloom syndrome.

Research Area

Cell Biology, Molecular Biology

Key Properties

• CAS Number: 1430213-30-1
• MW: 383.32
• Purity: 98.12%
• Formula: C₁₅H₉F₄N₅OS
• SMILES: Fc1ccc(NC(=O)Nc2nnc(s2)-c2ccncc2)cc1C(F)(F)F
• Target: DNA/RNA Synthesis
• Solubility: DMSO:5 mg/mL (13.04 mM)

Bioactivity

• Target IC50:
  BLM:1.8 μM
• In Vitro:
  ML216 shows cell-based activity and can induce sister chromatid exchanges, enhance the toxicity of aphidicolin, and exert antiproliferative activity in cells expressing BLM, but not those lacking BLM .
• Cell Research:
  Cells were treated with BLM inhibitors at the indicated concentrations for up to 72 hr. At each time point, the WST-1 reagent (a pale tetrazolium derivative converted to an intensely-colored formazan product by the action of mitochondrial dehydrogenases) was added, and after a 4 hr incubation the plates were analyzed optically at 450 nm with a reference wavelength of 690 nm .

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, ML216, ML-216, ML 216, Inhibitor, helicase, DNA, DNA/RNA Synthesis, DNA Synthesis, DNASynthesis, chromatid, CID 49852229, CID49852229, CID-49852229, BLM helicase, BLM, anti-proliferative, Anticancer, sister, WRN, RNASynthesis, RNA Synthesis, toxicity, unwinding

Compound Identifiers

PubChem CID

49852229