Mitochonic acid 5

SKU: orb1298996

Description

Mitochonic acid 5 (MA-5) is a synthetic derivative of indole-3-acetic acid that targets mitochondria, demonstrating protective effects in models of renal and cardiac injury. Research in vitro shows it enhances survival in patient-derived fibroblasts with mitochondrial disorders, while in vivo studies indicate benefits for ischemia-reperfusion damage.

Research Area

Cell Biology, Metabolism Research

Key Properties

• CAS Number: 1354707-41-7
• MW: 329.3
• Purity: 99.91% (May vary between batches)
• Formula: C₁₈H₁₃F₂NO₃
• SMILES: OC(=O)C(CC(=O)c1ccc(F)cc1F)c1c[nH]c2ccccc12
• Target: Mitochondrial Metabolism,OXPHOS
• Solubility: DMSO:34.29 mg/mL (104.13 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (10.02 mM)

Bioactivity

• In Vitro:
  Treatment with MA-5 reduced mitochondrial apoptosis via upregulation of mitophagy. Increased mitophagy degraded damaged mitochondria, disrupting mitochondrial apoptosis, neutralizing ROS overproduction, and improving cellular energy production. We also identified that MA-5 regulated mitophagy via Bnip3 through the MAPK-ERK-Yap signaling pathway. Inhibiting this signaling pathway or knocking down Bnip3 expression prevented MA-5 from having beneficial effects on mitochondrial homeostasis and increased microglial apoptosis.
• Cell Research:
  TNFα was used to induce inflammatory injury in mouse microglial BV-2 cells with and without prior MA-5 treatment. Cellular apoptosis was assessed using the MTT and TUNEL assays. Mitochondrial functions were evaluated via mitochondrial membrane potential JC-1 staining, ROS flow cytometry analysis, mPTP opening assessment, and immunofluorescence of cyt-c. Mitophagy was examined using western blots and immunofluorescence. The pathways analysis was carried out using western blots and immunofluorescence with a pathway blocker.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Inhibitor, MitochondrialMetabolism, Mitochonic acid 5, Mitochonic acid5, Mitochonic acid-5, Mitochondrial Metabolism, MA 5, MA5, MA-5, inhibit

Compound Identifiers

PubChem CID

76070959