Minocycline HCl

SKU: orb1309771

Description

Minocycline hydrochloride is a broad-spectrum tetracycline antibiotic with favorable pharmacokinetics, including high bioavailability and tissue penetration. It is widely used in research models of bacterial infection and acne, as well as in studying neuroinflammation and matrix metalloproteinase inhibition in both in vitro and in vivo experimental systems.

Research Area

Cell Biology, Immunology & Inflammation, Neuroscience, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 13614-98-7
• MW: 493.95
• Purity: >98%
• Formula: C₂₃H₂₇N₃O₇ · HCl
• SMILES: Cl.[C@@H]12[C@](C(=C3[C@H](C2)Cc2c(C3=O)c(ccc2N(C)C)O)O)(C(=O)C(=C([C@H]1N(C)C)O)C(=O)N)O
• Target: PARP
• Solubility: Soluble in DMSO (up to 50 mg/ml), or in Water (up to 10 mg/ml).

Bioactivity

• Target IC50:
  NO production (LPS-induced BV2 cells):9.07 ± 0.86 μM|NO production (LPS-induced RAW246.7 macrophages):34.81 μM|Ih amplitude:41 μM|MCF-7 cells viability:36.10 µM
• In Vivo:
  Minocycline inhibits the release of cytochrome c mediated by the permeability transition in mitochondria. This inhibition by minocycline on cytochrome c release has been demonstrated in vivo, in cells, and isolated mitochondria. Additionally, minocycline suppresses the activity of inducible caspase-1 and caspase-3, inducible nitric oxide synthase (iNOS), and p38 mitogen-activated protein kinase (MAPK). Following experimental focal ischemia, minocycline reduces the upregulation of caspase-1 and iNOS, thereby decreasing infarct size.
• In Vitro:
  Minocycline exhibits significant neuroprotective effects in models of cerebral ischemia, traumatic brain injury, Huntington's disease, and Parkinson's disease. Its neuroprotective properties may involve the inhibition of 5-lipoxygenase (an inflammatory enzyme associated with brain aging). Additionally, Minocycline's activity is linked to the inhibition of protein synthesis.

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

cerebral ischemia, Apoptosis, Antibiotic, Bacterial, Ca channels, Ca2+ channels, Calcium Channel, acinetobacter, Hypoxia-inducible factors, HIFs, HIFProlylHydroxylase, HIF-PH, HIF/HIFProlylHydroxylase, HIF/HIF ProlylHydroxylase, HIF/HIF Prolyl-Hydroxylase, HIF ProlylHydroxylase, HIF Prolyl-Hydroxylase, HIF, MDM-2/p53, ovarian cancer, Minocycline, Minocycline HCl, Minocycline hydrochloride, Minocycline Hydrochloride, Mitochondrial ATP-sensitive potassium, neuroprotective, Inhibitor, L-type Ca2+ channel, Myocardial ischemia, infection, KcsA, inhibit, stroke, PI3K/Akt, Potassium Channel, Ventricular arrhythmia

Compound Identifiers

PubChem CID

54685925