MID-1

SKU: orb1219463

Description

MID-1 is an inhibitor of MG53-IRS-1 (Mitsugumin 53-Insulin Receptor Substrate-1) interaction. It disrupts molecular association of MG53 with IRS-1 and abolishes MG53-induced IRS-1 ubiquitination and degradation in skeletal muscle, leading to elevated IRS-1 expression level and increased insulin signaling and glucose uptake.

Key Properties

• CAS Number: 312608-54-1
• MW: 293.3
• Purity: >98% (HPLC)
• Formula: C₁₂H₁₁N₃O₄S
• SMILES: CCOc1ccc(cc1)C(=O)Nc1ncc(s1)[N+]([O-])=O
• Target: MG53-IRS-1
• Solubility: DMSO:31.25 mg/mL (106.55 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  MID-1 does not have good pharmacokinetics In vivo.
• In Vitro:
  MID-1 (5 μM; 24 h) increases the IRS-1 expression level in skeletal muscle by disrupting the MG53-IRS-1 interaction. MID-1 (10 μM; 12 h) reduces the luciferase activity in HEK 293 cell line expressing NLUC-IRS-1 and CLUC-C14A. MID-1 (1-20 μM; 12 h) disrupts the MG53-IRS-1 interaction but not MG53-FAK interaction in HEK 293 cells. MID-1 (0.1-10 μM; 4-24 h) abolishes MG53-induced IRS-1 ubiquitination and degradation in HEK 293 cells. MID-1 (5-10 μM; 24 h) increases insulin signaling and insulin-elicited glucose uptake in C2C12 myotubes. MID-1 (5-10 μM; 24 h) enhances skeletal myogenesis. Western blot analysis. Cell line: C2C12 myotubes. Concentration: 5 μM. Incubation time: 24 h. Result: Increased the IRS-1 protein level.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

MG53-IRS-1