Micheliolide

SKU: orb1301582

Description

Micheliolide

Research Area

Immunology & Inflammation, Neuroscience, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 68370-47-8
• MW: 248.32
• Purity: 99.37%
• Formula: C₁₅H₂₀O₃
• SMILES: CC1=C2CC[C@@](C)(O)[C@@H]2[C@H]2OC(=O)C(=C)[C@@H]2CC1
• Target: NF-κB,NOS,TGF-beta/Smad,PI3K,COX,Akt
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (8.05 mM);DMSO:250 mg/mL (1006.77 mM)

Bioactivity

• In Vitro:
  Treatment of BV2 cells with Micheliolide(MCL) significantly repressed LPS-stimulated nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression, as well as tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and nitric oxide (NO) induction. MCL also attenuated mRNA levels of multiple pro-inflammatory cytokines and mediators such as iNOS, COX-2, TNF-α, IL-6 and IL-1β. Mechanistic studies revealed that MCL suppressed LPS-stimulated the activation of IκBα/NF-κB pathway and Akt pathway. Moreover, MCL inhibited LPS-induced the activition of c-Jun N-terminal kinase (JNK), p38 MAPK kinase, and extracellular signal-regulated kinases 1/2 (ERK1/2). Meanwhile, MCL markedly promoted antioxidant protein heme oxygenase-1 (HO-1) expression by enhancing NF-E2-related factor 2 (Nrf2) activity. Together, MCL may serve as a neuroprotective agent in neuroinflammation-related neurodegenerative disorders.
• Cell Research:
  BV2 cells were pretreated with the indicated concentrations of Micheliolide 1 h prior to stimulation with LPS (1 μg/ml) for 6 h and total RNA was extracted using RaPure Total RNA Kit.Reverse transcription was performed using RevertAid First Strand cDNA Synthesis Kit. Real-time PCR analysis was performed using SYBR Select Master Mix in conjunction with an ABI Prism 7500 Sequence Detection System with the expression of β-actin as the internal control. The data were analyzed using the ΔΔCT method. The primers for iNOS, COX-2, TNF-α, IL-1β, IL-6 and β-actin were described as below.

Storage & Handling

• Storage: keep away from direct sunlight,keep away from moisture | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Nuclear factor-kappaB, Nuclear factor-κB, NF-κB, NFκB, NFkB, NF-kB, Micheliolide, inhibit, Inhibitor, Inos, COX-2

Compound Identifiers

PubChem CID

442279