Micafungin sodium

SKU: orb1307431

Description

Micafungin sodium is a semi-synthetic echinocandin antifungal agent derived from Coleophoma empetri. It is widely used in research to study invasive fungal infections, demonstrating potent activity against Candida and Aspergillus species in both in vitro assays and in vivo animal models.

Research Area

Infectious Disease & Virology

Key Properties

• CAS Number: 208538-73-2
• MW: 1292.26
• Purity: >99.99% (May vary between batches)
• Formula: C₅₆H₇₀N₉NaO₂₃S
• SMILES: [Na+].[H][C@@]12C[C@@H](O)CN1C(=O)[C@@]([H])(NC(=O)[C@H](C[C@@H](O)[C@@H](O)NC(=O)[C@]1([H])[C@@H](O)[C@@H](C)CN1C(=O)[C@@]([H])(NC(=O)[C@@]([H])(NC2=O)[C@H](O)[C@@H](O)c1ccc(O)c(OS([O-])(=O)=O)c1)[C@H](O)CC(N)=O)NC(=O)c1ccc(cc1)-c1cc(on1)-c1ccc(OCCCCC)cc1)[C@@H](C)O
• Target: Antibiotic,Antifungal
• Solubility: Ethanol:< 1 mg/mL (insoluble or slightly soluble);DMSO:242 mg/mL (187.27 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5 mg/mL (3.87 mM);H2O:100 mg/mL (77.38 mM)

Bioactivity

• In Vivo:
  Micafungin administration at a dose of 1 mg/kg notably extends survival in mice compared to those receiving saline. Additionally, a regimen combining micafungin (0.1 mg/kg) with KB425796-C (32 mg/kg) appears to enhance survival duration when compared to treatment with only micafungin (0.1 mg/kg). Treatment with micafungin alone reduces colony-forming units (CFUs) in the liver, though its clearance efficacy is not as pronounced as in the kidney. Significantly, a combined treatment of micafungin and KB425796-C markedly lowers CFU counts across all tested doses compared to micafungin treatment alone, demonstrating a superior clearance effect than that seen with AMPH-treated animals.
• In Vitro:
  Micafungin (10 mg/mL) effectively inhibits biofilm formation in the majority of examined isolates and significantly reduces mRNA transcription levels across all tested genes compared to untreated samples. Additionally, when combined with KB425796-C, micafungin exhibits a fungicidal effect, significantly diminishing the colony-forming unit (CFU) count, unlike the fungistatic outcome (no CFU reduction) observed when either drug is applied independently.
• Cell Research:
  Each fungal isolate is incubated statically in yeast-maltose (YM) agar broth for 24 h at 30°C. Cryptococcus neoformans YC203 is grown in YM broth medium for 20 h at 30°C with shaking at 200 r.p.m. A cell suspension is prepared by washing the cultured cells once with sterile saline. A. fumigatus FP1305 is cultured on a potato dextrose agar (PDA) slant for 4 days, and spores are then harvested in sterile saline and collected by filtering through gauze. Antifungal activity against all isolates, with the exception of C. neoformans, is measured by the micro-broth dilution method in 96-well culture plates using RPMI 1640 medium supplemented with l-glutamine, but without sodium bicarbonate, and buffered to pH 7.0 with 0.165 m MOPS. ForC. neoformans, yeast nitrogen base-glucose (YNBD) medium is used. For the assay, the test microorganism is inoculated into each well to yield 1×105 CFU/well, and the plates are then incubated for 20 h or 48 h at 37°C. Two end points are determined by microscopic observation: MEC, which is defined as a substantial reduction in fungal growth, and MIC, which is defined as a complete inhibition of growth.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Antibiotic, 1,3-beta-D-glucan synthesis, Micafungin, Micafungin sodium, Inhibitor, inhibit, Mycamine Sodium, Fungal, FK463, FK-463, FK463 Sodium, FK 463

Compound Identifiers

PubChem CID

23666118