MHY1485

SKU: orb1225304

Description

MHY1485 is a mTOR activator; inhibits the autophagic process by inhibition of fusion between autophagosomes and lysosomes leading to the accumulation of LC3II protein and enlarged autophagosomes.(In Vitro):MHY1485 (10 μM; 4 hours) shows that GCDC-induced autophagic activity is inhibited by upregulating p-mTOR expression and downregulating LC3 and p62 expression in HCC cells.MHY1485 (5 μM; 6 hours) increases the LC3II/LC3I ratio in a dose and time-dependently manner due to presumably inhibited LC3II degradation in rat liver Ac2F cells.MHY1485 (0.5-2 μM; 6 hours) increases the phosphorylation of mTOR at ser2448 and upregulates the level of phosphorylation of 4E-BP1 in a dose-dependently manner in Ac2F cells.(In Vivo):MHY1485 (intraperitoneal injection; 10 mg/kg, 2 days) blocks the autophagy signaling induced by follicle-stimulating hormone (FSH). It increases p-mTOR and p-S6K1 expression levels, whereas LC3 expression shows no marked change compared to that in the control group.

Key Properties

• CAS Number: 326914-06-1
• MW: 387.39
• Purity: >98% (HPLC)
• Formula: C₁₇H₂₁N₇O₄
• SMILES: O=[N+](C1=CC=C(NC2=NC(N3CCOCC3)=NC(N4CCOCC4)=N2)C=C1)[O-]
• Target: mTOR
• Solubility: DMSO: 33 mg/mL (85.18 mM)

Bioactivity

• In Vivo:
  MHY1485 (intraperitoneal injection; 10 mg/kg, 2 days) blocks the autophagy signaling induced by follicle-stimulating hormone (FSH). It increases p-mTOR and p-S6K1 expression levels, whereas LC3 expression shows no marked change compared to that in the control group. Animal model: 4-week-old female ICR mice. Dosage: 10 mg/kg, 2 days. Administration: Intraperitoneal injection. Result: Suppressed the autophagy level following FSH treatment.
• In Vitro:
  MHY1485 (10 μM; 4 hours) shows that GCDC-induced autophagic activity is inhibited by upregulating p-mTOR expression and downregulating LC3 and p62 expression in HCC cells. MHY1485 (5 μM; 6 hours) increases the LC3II/LC3I ratio in a dose and time-dependently manner due to presumably inhibited LC3II degradation in rat liver Ac2F cells. MHY1485 (0.5-2 μM; 6 hours) increases the phosphorylation of mTOR at ser2448 and upregulates the level of phosphorylation of 4E-BP1 in a dose-dependently manner inAc2F cells. Western blot analysis. Cell line: HCC cells. Concentration: 10 μM. Incubation time: 4 hours. Result: Upregulated p-mTOR and downregulated LC3 and p62 expression.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MHY-1485 | MHY1485 | MHY 1485

Quick Database Links

Gene Symbol

mTOR