Methyl Green

• Catalog Number: orb2652918
• Category: Tools
• Description: This methyl green counterstain solution is designed for nuclear counterstain after completion of Immunohistochemistry staining or for routine histology.
• Tested applications: IHC
• Application notes: A. Immunohistochemistry: 1. After chromogen staining, wash slide 2-3X with de/di water, 2X with buffer and 2X with de/di. water 2. Apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes). 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (limonene) or equivalent. B. Histology: A. Follow the procedure as outlined for hitology 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. At the counterstain step, apply Methyl Green counterstain for 3-5 minutes or longer (if deep green color is required, slide may be incubated at 60ºC for 2-5 minutes). 3. DO NOT rinse with water. Go directly into 95 % alcohol for few dips and 100% alcohol 5-7X. 4. Clear with Xylene or clearing agent. 5. The slides can be mounted with Organic mounting mediums like Organo mount (ImmunoBioscience cat. # AR-6504) or equivalent.
• Storage: Room Temperature
• Note: For research use only