Methyl-β-cyclodextrin

SKU: orb1303195

Description

Methyl-β-cyclodextrin is a macrocyclic solubilizing agent for hydrophobic compounds. It functions as a cholesterol-depleting lipid raft disruptor, a property utilized in cell biology research to study membrane dynamics and signaling pathways in both in vitro and in vivo experimental models.

Key Properties

• CAS Number: 128446-36-6
• MW: 1310(Average)
• Purity: 99.45% (May vary between batches)
• Formula: C₅₄H₉₄O₃₅
• SMILES: COCC1OC2OC3C(COC)OC(OC4C(COC)OC(OC5C(COC)OC(OC6C(COC)OC(OC7C(COC)OC(OC8C(COC)OC(OC1C(O)C2OC)C(OC)C8O)C(OC)C7O)C(OC)C6O)C(OC)C5O)C(OC)C4O)C(OC)C3O
• Target: Others
• Solubility: DMSO:55 mg/mL

Bioactivity

• Target IC50:
  PEL:3.33-4.23 mM.|LPS/oxLDL-triggered monocyte-endothelial adhesion and related molecules (signalling pathway):27.66 mM|CHO cellslesterol:14.4 μM
• In Vivo:
  METHODS: To test the antitumor activity in vivo, Methyl-β-cyclodextrin (500 mg/kg) was intraperitoneally injected into NRJ mice bearing PEL tumor BCBL-1 once a day for twenty-one days. RESULTS: Methyl-β-cyclodextrin significantly inhibited the growth and invasion of PEL cells without significant adverse effects. METHODS: To investigate the modulatory effects on collagen, Methyl-β-cyclodextrin (1.25-5.0 mg/mouse) was intradermally injected into the SKH1 mouse twice a week for two months. RESULTS: Methyl-β-cyclodextrin showed potent COL I up-regulatory activity resulting in increased skin thickness.
• In Vitro:
  METHODS: Five primary exudative lymphoma (PEL) cell lines, BCBL-1, BC-1, BC-3, TY-1, and GTO, were treated with Methyl-β-cyclodextrin (0-10 mM) for 24 h, and cell viability was measured by MTT assay. RESULTS: Methyl-β-cyclodextrin dose-dependently inhibited the growth of PEL cells with IC50 between 3.33-4.23 mM. METHODS: Chicken hepatocellular carcinoma cells, LMH, were treated with Methyl-β-cyclodextrin (10 mM) for 1 h, then cholesterol (50 µg/mL) was added at different time points before, during, and after infection with FAdV-4, and the expression levels of the target proteins were detected by Western Blot. RESULTS: When LMH cells were pretreated with increasing concentrations of Methyl-β-cyclodextrin prior to infection, penton protein levels decreased in a dose-dependent manner.
• Cell Research:
  PEL cells are incubated in triplicate in a 96-well microculture plate in the presence of different concentrations of methyl-β-cyclodextrin (0-10 mM) in a final volume of 0.1 mL for 24 h at 37°C. Subsequently, MTT (0.5 mg/mL final concentration) is added to each well. After 3 h of additional incubation, 100 μL of a 0.04 N HCl is added to dissolve the crystals. Absorption values at 570 nm are determined.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

beta-Cyclodextrin methyl ethers, b-Cyclodextrin methyl ethers, Methyl β cyclodextrin, Methyl-b-cyclodextrin, Methyl-beta-cyclodextrin, Methylβcyclodextrin, Methyl-β-cyclodextrin, Inhibitor, inhibit, β-Cyclodextrin methyl ethers

Compound Identifiers

PubChem CID

122143