Methotrexate α-tert-butyl ester

SKU: orb3027140

Description

Methotrexate α-tert-butyl ester is a protected analog of the antineoplastic and immunosuppressive drug methotrexate (MTX), in which the β-carboxyl group of the L-glutamate moiety is esterified with a tert-butyl group. Methotrexate α-tert-butyl ester is primarily used in chemical synthesis and prodrug development studies.

Research Area

Cell Biology, Molecular Biology, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 79640-70-3
• MW: 510.555
• Purity: 98.62% (May vary between batches)
• Formula: C₂₄H₃₀N₈O₅
• SMILES: NC=1C2=C(N=C(N)N1)N=CC(CN(C)C3=CC=C(C(N[C@H](C(OC(C)(C)C)=O)CCC(O)=O)=O)C=C3)=N2
• Target: Others,Antifolate,DNA/RNA Synthesis
• Solubility: DMSO:80 mg/mL

Bioactivity

• In Vivo:
  In HT1080 tumor-bearing mice, Methotrexate α-tert-butyl ester significantly inhibits tumor growth. This finding suggests that protecting the α-carboxyl group of methotrexate can enhance the circulatory half-life and minimize liver accumulation for similar dendrimer conjugates without compromising their in vivo antitumor efficacy.
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• In Vitro:
  MTX-PVG exhibits activity against HT1080 cells, the OtBu-MTX-PVG derivative(Methotrexate α-tert-butyl ester) is less active, indicating that protecting the α-carboxyl group of MTX Results in decreased in vitro activity.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Others,Antifolate,DNA/RNA Synthesis