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Catalog Number | orb1231018 |
---|---|
Category | Proteins |
Description | MERS Spike S1 Recombinant protein |
Tag | His Tag |
Form/Appearance | Liquid |
Purity | > 95% pure as determined by 12% PAGE (coomassie staining). |
Protein Sequence | Recombinant SARS MERS Spike S1 is a peptide from amino acids 56-295 of spike protein S1 produced in E. coli and fused to a 6xHis tag at its C-terminus (UniProt accession #AHC74088).SARS MERS is purified by a proprietary chromatographic technique. |
Source | E. coli |
Storage | Store in working aliquots at -20˚°C; For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid freeze/thaw cycles. |
Buffer/Preservatives | SARS MERS protein solution is supplied in PBS, 25mM arginine and 0.05% sodium azide. |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
ELISA, WB | |
> 95% as determined by SDS-PAGE. | |
83.1 kDa | |
HEK293 cells |
98.00% | |
142.52 kDa (predicted); 157.6 kDa (reducing conditions) |
Greater than 85.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
93.30% | |
79.9 kDa (predicted); 94 kDa (reducing condition, due to glycosylation) |