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Catalog Number | orb700631 |
---|---|
Category | Proteins |
Description | MERS S1 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Arg 751 (Accession # K0BRG7-1). |
Reactivity | Virus |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 83.1 kDa |
Target | Spike S1 |
UniProt ID | K0BRG7 |
Protein Sequence | Tyr 18 - Arg 751 |
Source | MERS S1 protein, His Tag (orb700631) is expressed from human 293 cells (HEK293). It contains AA Tyr 18 - Arg 751 (Accession # K0BRG7-1). |
Expression System | HEK293 |
Biological Origin | MERS |
Expression Region | Tyr 18 - Arg 751 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | Spike,S1 protein,Spike glycoprotein Subunit1,S gly Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 83.1 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
98.00% | |
142.52 kDa (predicted); 157.6 kDa (reducing conditions) |
Greater than 85.0% as determined by SDS-PAGE. | |
Sf9, Baculovirus cells |
93.30% | |
79.9 kDa (predicted); 94 kDa (reducing condition, due to glycosylation) |
82.90% | |
44.10 kDa (predicted) |
> 95% by SDS-PAGE. | |
Recombinant MERS-CoV Spike RBD Protein with His Tag is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Glu367-Tyr606) of mers-cov Spike RBD (Accession #AFS88936.1) fused with an 6��His Tag at the C-terminus. |