Melatonin

SKU: orb1307851

Description

Melatonin is an endogenous hormone produced by the pineal gland that acts on melatonin receptors to regulate circadian rhythms. It is widely used in research on sleep disorders, chronobiology, and neuroprotection, with applications in both in vitro assays and in vivo animal studies due to its antioxidant and anti-inflammatory properties.

Research Area

Cell Biology, Metabolism Research, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 73-31-4
• MW: 232.28
• Purity: 99.89% (May vary between batches)
• Formula: C₁₃H₁₆N₂O₂
• SMILES: COC1=CC=C2NC=C(CCNC(C)=O)C2=C1
• Target: Autophagy,Glutathione Peroxidase,CaMK,ROR,Estrogen/progestogen Receptor,Mitophagy,Melatonin Receptor,Apoptosis,Endogenous Metabolite
• Solubility: 10% DMSO+90% Saline:1.16 mg/mL (4.99 mM);DMSO:55 mg/mL (236.78 mM)

Bioactivity

• In Vivo:
  METHODS: To assay in vivo activity, EIPA (10 mg/kg) was injected intraperitoneally into BALB/c mice bearing 4T1 xenografts, and 70 kD FITC-Ficoll was injected into the tumors 1 h later. The mice were necropsied 1 h after Ficoll injection, and the tumors were excised and frozen in OCT. RESULTS: EIPA-sensitive 70 kD FITC-Ficoll uptake was observed in in situ homozygous 4T1 tumors of BALB/c mice, suggesting that AMPK activation or other signals are sufficient to trigger the formation of large fusions in vivo.
• In Vitro:
  METHODS: Ten cells were pretreated with EIPA (50 µM) for 1.5 h. Dextran index was measured by Dextran uptake assay. RESULTS: Immortalized but untransformed hTERT-HME1 mammary epithelial cells and MCF10A cells did not exhibit megaloblast efflux in complete medium but stimulated dextran uptake by nutrient deprivation. Although PIK3CB was found to be required for growth factor-stimulated macrocytosis, oncogenic mutations in PIK3CA were sufficient to induce constitutive macrocytosis in mouse embryonic fibroblast MEFs and untransformed MCF10A cells. METHODS: MKN28 cells were treated with EIPA (5-100 µM) for 48 h. Cell proliferation was detected by cell count. RESULTS: Cell exposure to EIPA inhibited the proliferation of MKN28 cells in a dose- and time-dependent manner.

Storage & Handling

• Storage: store at low temperature,keep away from direct sunlight,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Autophagy, CAM, Endogenous Metabolite, N-Acetyl-5-methoxytryptamine, Mitophagy, Mitochondrial Autophagy, MPO, inhibit, Melatonin Receptor, melatonin receptor, Melatonin, MelatoninReceptor, Melatonine, Inhibitor, ER, EndogenousMetabolite, GlutathionePeroxidase, Glutathione Peroxidase, Estrogen Receptor, EstrogenReceptor, progestogen Receptor, RORβ, progestogenReceptor

Compound Identifiers

PubChem CID

896