ME0328

SKU: orb1300642

Description

ME0328

Research Area

Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 1445251-22-8
• MW: 321.37
• Purity: 99.84%
• Formula: C₁₉H₁₉N₃O₂
• SMILES: C[C@H](NC(=O)CCc1nc2ccccc2c(=O)[nH]1)c1ccccc1
• Target: PARP
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.22 mM);H2O:< 1 mg/mL (insoluble or slightly soluble);DMSO:60 mg/mL (186.7 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

Bioactivity

• Target IC50:
  PARP3:0.89 μM|ARTD2/PARP2:10.8 μM|ARTD10/PARP10:71.3 μM|ARTD6/TNKS2:34.3 μM|ARTD5/TNKS1:47.3 μM|ARTD1/PARP1:6.3 μM
• In Vitro:
  ME0328 is soluble, cell permeable, and metabolically stable in human liver microsomes and rat hepatocytes. ME0328 (10 μM) results in a significant delay of γH2AX-foci resolution by affecting ARTD3 in A549 and MRC5 cells without significant toxicity.
• Cell Research:
  Compound cytotoxicity in A549 and MRC5 cells is evaluated using WST-1 assays. A549 cells are cultured in Dulbecco’s Modified Eagle’s Medium supplemented with 10% fetal calf serum (FCS), penicillin, and streptomycin. MRC5 cells are cultured in Minimal Essential Medium supplemented with 10% FCS, penicillin, streptomycin, and l-glutamine. Both cell lines are maintained in a humidified incubator at 37°C and 5% CO2. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Disclaimer: For research use only

Alternative Names

PARP, PARP3, PARP1, inhibit, Inhibitor, ME0328, ME-0328, ME 0328, poly ADP ribose polymerase

Compound Identifiers

PubChem CID

135566764