MCC950

SKU: orb1693248

Description

MCC950 (CP-456773) is a potent and selective NLRP3 inflammasome inhibitor with an IC50 in the low nanomolar range. It is widely used in research models of inflammatory diseases, autoimmunity, and neurological disorders, demonstrating efficacy in both in vitro cellular assays and in vivo animal studies.

Research Area

Immunology & Inflammation, Signal Transduction

Key Properties

• CAS Number: 210826-40-7
• MW: 404.48
• Purity: 99.89% (May vary between batches)
• Formula: C₂₀H₂₄N₂O₅S
• SMILES: CC(C)(O)c1coc(c1)S(=O)(=O)NC(=O)Nc1c2CCCc2cc2CCCc12
• Target: NOD,NOD-like Receptor (NLR)
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:3.3 mg/mL (8.16 mM);DMSO:83.33 mg/mL (206.02 mM)

Bioactivity

• Target IC50:
  BMDMs:7.5 nM|HMDM:8.1 nM
• In Vivo:
  METHODS: To study the anti-inflammatory effect of MCC950, mice were pre-treated with MCC950 for 1 hour and then intraperitoneally injected with LPS. RESULTS: It was detected 2 hours later that MCC950 could reduce the concentrations of IL-1β and IL-6 in serum, but had little effect on the amount of TNF-α, indicating that MCC950 was active in vivo. METHODS: To study the anti-inflammatory effect of MCC950, 7-week-old Winnie mice (with Muc2 gene mutation and C57BL/6J background) were used and divided into the MCC950 treatment group and the control group, with 10 mice in each group. The mice in the treatment group were fed fresh feed paste containing 40mg/kg MCC950 every day, while the control group was fed feed paste without MCC950. The experiment lasted for 21 days. RESULTS: The DAI score of mice in the MCC950 treatment group was significantly reduced, indicating that the consistency of their feces and the blood conditions in the feces were improved. The colonic length of mice in the treatment group increased and the colonic weight decreased, indicating that colonic inflammation was alleviated. The H&E staining RESULTS showed that the colonic inflammation characteristics of mice in the MCC950 treatment group were significantly improved. METHODS: To study the effect of MCC950 on non-obese diabetes, MCC950 was intraperitoneally injected into 11-week-old female non-obese diabetic (NOD) mice three times a week for three consecutive weeks. RESULTS: MCC950 treatment led to a significant reduction in salivary secretion and exacerbated the infiltration of white blood cells in the submandibular gland. In addition, it is accompanied by an increase in the number of T cells and B cells, an enhanced Th1 response, and a decrease in the expression of aquaporin 5 in the submandibular gland.
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• In Vitro:
  METHODS: HEK293 cells and HepG2 cells were treated with MCC950 and cytotoxicity was detected by Resazurin-based fluorescence assay. RESULTS: MCC950 is non-toxic to HEK293 cells (CC50> 62.5 μM) and HepG2 cells (CC50> 62.5 μM). METHODS: Human THP-1 cells were treated with MCC950 for 72 hours and the cytotoxicity was detected by the CCK-8 method. RESULTS: MCC950 inhibited the growth of THP-1 cells (CC50=81.35 μM). METHODS: Human THP-1 cells were treated with MCC950 for 72 hours and then cytotoxicity was detected by the MTT method. RESULTS: MCC950 inhibited the growth of THP-1 cells (IC50=98.83 μM).
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• Cell Research:
  MCC950 is dissolved in DMSO and stored, and then diluted with appropriate media before use. BMDM are seeded at 5×105/mL or 1×106/mL, HMDM at 5×105/mL and PBMC at 2×106/mL or 5×106/mL in 96 well plates. The following day the overnight medium is replaced and cells are stimulated with 10 ng/mL LPS from Escherichia coli serotype EH100 (ra) TLRgrad for 3 h. Medium is removed and replaced with serum free medium (SFM) containing DMSO (1:1,000), MCC950 (0.001-10 μM), glyburide (200 μM), Parthenolide (10 μM) or Bayer cysteinyl leukotriene receptor antagonist 1-(5-carboxy-2{3-[4-(3-cyclohexylpropoxy)phenyl]propoxy}benzoyl)piperidine-4-carboxylic acid (40 μM) for 30 min. Cells are then stimulated with inflammasome activators: 5 mM adenosine 5'-triphosphate disodium salt hydrate (ATP) (1 h), 1 μg/mL Poly(deoxyadenylic-thymidylic) acid sodium salt (Poly dA:dT) transfected with Lipofectamine 200 (3-4 h), 200 μg/mL MSU (overnight) and 10 μM nigericin (1 h) or S. typhimurium UK-1 strain. Cells are also stimulated with 25 μg/mL Polyadenylic-polyuridylic acid (4 h). For non-canonical inflammasome activation cells are primed with 100 ng/mL Pam3CSK4 for 4 h, medium is removed and replaced with SFM containing DMSO or MCC950 and 2 μg/mL LPS is transfected using 0.25% FuGENE for 16 h. Supernatants are removed and analysed using ELISA kits. LDH release is measured using the CytoTox96 non-radioactive cytotoxicity assay.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRID 3, CRID3, CRID-3, CP456773, CP-456773, CP 456773, MCC-950, MCC 950, MCC950, Inhibitor, inhibit, NOD-like Receptor (NLR), NLRP3

Compound Identifiers

PubChem CID

9910393