MARCKS Antibody

SKU: orb1253694

Description

MARCKS Antibody

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Application Notes:
  Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: MARCKS (phospho-Ser170) antibody was raised against a peptide sequence around phosphorylation site of Serine 170 (G-F-S (p) -F-K) derived from Human MARCKS.
• Target: MARCKS
• Molecular Weight: 80 kDa
• Purification: Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MACS, 80K-L, PKCSL, PRKCSL, HSP27, HSP28, Protein kinase C substrate, 80 kDa protein, light chain

Western blot analysis of lysed extracts from MCF cells untreated or treated with EGF using MARCKS (phospho-Ser170).

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using MARCKS (Phospho-Ser170).

Quick Database Links

Gene Symbol

MARCKS

UniProt

P29966

RefSeq (Protein):NP_002347.5