Malachite green oxalate

SKU: orb1297705

Description

Malachite green oxalate is a triphenylmethane dye widely employed as a colorimetric reagent for phosphate detection. It is utilized in enzymatic assays, such as those for phosphatases and kinases, enabling quantitative analysis of phosphate release in both in vitro biochemical research and high-throughput screening applications.

Research Area

Cell Biology, Infectious Disease & Virology, Signal Transduction

Key Properties

• CAS Number: 2437-29-8
• MW: 463.51
• Formula: C₂₃H₂₅N₂·C₂HO₄·1/2C₂H₂O₄
• SMILES: O=C(C(O)=O)[O-].O=C(C(O)=O)O.CN(C)C(C=C1)=CC=C1/C(C2=CC=CC=C2)=C3C=C/C(C=C/3)=[N+](C)/C.O=C(C(O)=O)[O-].CN(C)C(C=C4)=CC=C4/C(C5=CC=CC=C5)=C6C=C/C(C=C/6)=[N+](C)/C
• Target: Apoptosis,IκB/IKK,Antifungal,NF-κB
• Solubility: DMSO:8.67 mg/mL (18.71 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:0.5 mg/mL (1.08 mM)

Bioactivity

• In Vitro:
  Bacterial staining experiment Material preparation: Malachite green oxalate stain, bacterial smear to be tested, alcohol lamp, distilled water, safranin stain, etc. Experimental steps: a. Make a bacterial smear, dry it naturally and fix it with the flame of an alcohol lamp. b. Add Malachite green oxalate stain, cover the smear, heat it until steam comes out, keep it for about 5 minutes, add appropriate stain to prevent drying, and then rinse with distilled water. c. Re-stain with safranin stain for 2-3 minutes, rinse with distilled water and dry. d. Observe under a microscope, the bacteria are dyed green, and structures such as spores can be clearly observed.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

antitumor, Apoptosis, enzymatic, I kappa B kinase, Inhibitor, Malachite green, Malachite green oxalate, Malachite green Oxalate, p65, Nuclear factor-kappaB, Nuclear factor-κB, NFκB, NFkB, NF-kB, NF-κB, inhibit, IRF3, IκBα, IκB kinase, IκB, IkB, IkB/IKK, IKBKE, IKK, phosphate

Compound Identifiers

PubChem CID

2724411