LY-294002 hydrochloride

SKU: orb1303185

Description

LY-294002 hydrochloride is a potent and cell-permeable inhibitor of phosphoinositide 3-kinase (PI3K) isoforms α, δ, and β. It is widely used in vitro to study PI3K signaling pathways in cancer and cellular metabolism, and it also inhibits autophagosome formation, facilitating autophagy research.

Research Area

Cell Biology, Metabolism Research, Molecular Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 934389-88-5
• MW: 343.81
• Purity: 99.98% (May vary between batches)
• Formula: C₁₉H₁₇NO₃·HCl
• SMILES: Cl.O=c1cc(oc2c(cccc12)-c1ccccc1)N1CCOCC1
• Target: Apoptosis,DNA-PK,Casein Kinase,PI3K
• Solubility: DMSO:50 mg/mL (145.43 mM);Ethanol:5 mM

Bioactivity

• Target IC50:
  PI3Kα:0.5μM|PI3Kβ:0.97 μM|CK2α2:3.869 μM|CK2:0.098 μM|PI3Kδ:0.57μM
• In Vivo:
  LY294002 can suppress tumor growth and induce apoptosis, especially in the LoVo tumors. Thus, It shows remarkable effectiveness in the mouse peritonitis carcinoma tosa model . LY294002 markedly inhibits growth and ascites formation of ovarian carcinoma .
• In Vitro:
  LY294002 inactivates Akt/PKB, consequently inhibiting cell proliferation and inducing apoptosis. In these colon cancer cell lines, LY294002 shows a remarkable growth-inhibitory and apoptosis-inducing effect and decreases expression of phosphorylated Akt (Ser473). LY294002 induces diminished cytoplasmic volume and obvious nuclear pyknosis in the tumor cells. LY294002 induces specific G1 arrest in cell growth, leading to almost complete inhibition of melanoma cell proliferation and partial inhibition of MG-63 (osteosarcoma cell line) proliferation.
• Cell Research:
  Cell lines: Colon cancer cell lines DLD-1,LoVo,HCT15,and Colo205. Concentrations: 0–50 μM. Incubation Time: 0–48 hours. Method: 1.0×105 cells (100 μL volume/well) are inoculated into 96-well microtiter plates.LY294002 is added to triplicate wells and cultured at 37oC for 0–48 hours.After treatment,Premix WST-1 (10 μL) is added to each microculture well,and the plates are incubated for 60 minutes at 37oC,after which absorbance at 450 nm is measured with a microplate reader.
• Animal Research:
  Animal Models: Two groups of athymic nude mice (5–7 weeks) are inoculated i.p.with OVCAR-3 cells. Formulation: Dissolved in DMSO plus 0.25 ml of PBS. Dosages: 0–100 mg/kg. Administration: i.p.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

spermatozoa, reversibly, phosphatidylinositol 3-kinase, SF1101, SF-1101, p110δ, p110β, p110α, tumor, translocation, CNE-2Z, broad-spectrum, Human, Leptin, LY 294002, LY 294002 hydrochloride, LY-294002, LY294002 hydrochloride, LY-294002 Hydrochloride, LY294002, Lysophosphatidic acid, nasopharyngeal, nuclear, NSC697286, NSC-697286, NSC 697286, NPC

Compound Identifiers

PubChem CID

11957589