LMK-235

SKU: orb1301108

Description

LMK-235 is a potent and selective HDAC inhibitor, primarily targeting HDAC4 and HDAC5. It is widely used in cancer research to study epigenetic mechanisms, demonstrating activity in both in vitro cell culture models and in vivo tumor studies.

Research Area

Epigenetics & Chromatin, Molecular Biology

Key Properties

• CAS Number: 1418033-25-6
• MW: 294.35
• Purity: 99.68% (May vary between batches)
• Formula: C₁₅H₂₂N₂O₄
• SMILES: Cc1cc(C)cc(c1)C(=O)NOCCCCCC(=O)NO
• Target: HDAC
• Solubility: DMSO:50 mg/mL (169.87 mM);Ethanol:29.4 mg/mL (99.88 mM);2% DMSO+40% PEG300+5% Tween-80+53% Saline:1 mg/mL (3.4 mM)

Bioactivity

• Target IC50:
  HDAC5:4.2 nM|HDAC2:881 nM|HDAC4:11.9 nM|HDAC1:320 nM|HDAC6:55.7 nM|HDAC8:1278 nM|HDAC11:852 nM
• In Vivo:
  LMK-235 demonstrates significant cytotoxicity against the breast cancer cell line MDA-MB-231, the oral cancer cell line Cal27, and the esophageal cancer cell line Kyse510, notably enhancing the cytotoxic effect of cisplatin. Additionally, LMK-235 exhibits nanomolar-level activity across various life-cycle stages of Plasmodium species. It induces histone deacetylase (HDAC) inhibition in human cancer cell lines with varying sensitivity to cisplatin compounds, achieving an IC50 of less than 1 μM.
• Cell Research:
  The rate of cell survival under the action of test substances is evaluated by an improved MTT assay. The assay is based on the ability of viable cells to metabolize yellow MTT to violet formazan that can be detected spectrophotometrically. In brief, A2780, Cal27, Kyse510, and MDA-MB-231 cell lines are seeded at a density of 5000, 7000, 8000, and 10 000 cells/well in 96-well plates. After 24 h, cells are exposed to increased concentrations of the test compounds. Incubation is ended after 72 h, and cell survival is determined by addition of MTT solution (5 mg/mL in phosphate buffered saline). The formazan precipitate is dissolved in DMSO. Absorbance s measured at 544 and 690 nm in a FLUOstar microplate reader. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, LMK 235, LMK235, LMK-235, Histone deacetylases, HDAC, HDAC5, HDAC4, Inhibitor

Compound Identifiers

PubChem CID

71520717