Llama IgG2 isotype control Antibody

SKU: orb343834

Description

Llama IgG2 isotype control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Dilution Range: ELISA: User Optimized, FC: User Optimized, WB: User Optimized
• Application Notes:
  Llama IgG2 isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG
• Purity: Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to llama IgG1 or IgG3 are observed.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.2 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Llama IgG2 isotype, Llama IgG2 subclass isotype, Llama IgG2a, IgG2b, IgG2c

SDS-PAGE of Llama IgG2 Isotype Control. Lane 1: Llama IgG1 (p/n orb343832), Non-reduced. Lane 2: Llama IgG2 (p/n orb343833), Non-reduced. Lane 3: Llama IgG3 (p/n orb343834), Non-reduced. M: 3 µl Opal Pre-stained Ladder. Lane 4: Llama IgG1, Reduced. Lane 5: Llama IgG2, Reduced. Lane 6: Llama IgG3, Reduced. Load: 1.0 µg per lane. Predicted/Observed size: Llama IgG2 80 kDa Non-reduced; 46 kDa Reduced.