Llama IgG2 isotype control Biotin Antibody

SKU: orb343843

Description

Llama IgG2 isotype control Biotin Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: DOT
• Dilution Range: ELISA: User Optimized, FC: User Optimized, WB: User Optimized
• Application Notes:
  Llama IgG2 Biotin conjugated isotype control has been tested by dot blot and can be utilized in Flow Cytometry, ELISA, and western blotting experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG
• Purity: Llama IgG2 isotype control (which consists of IgG2a, IgG2b and IgG2c isotypes) has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG2 shows a band of ~100 kDa whereas the reduced form exhibits ~46 kDa. No bands corresponding to unconjugated llama IgG1 or IgG3 are observed. Biotin Conjugated Llama IgG2 was proven by Dot Blot.
• Conjugation: Biotin

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Llama IgG2 isotype, Llama IgG2 subclass isotype, Llama IgG2a, IgG2b, IgG2c, Llama IgG2 Biotin Conjugated, Llama IgG2 BAC, Llama isotype control

Dot Blot Results of Llama IgG2 Isotype control Biotin Conjugated. Llama IgG2 Isotype control Biotin Conjugate (1) 100 ng, (2) 33.33 ng, (3) 11.11 ng, (4) 3.70 ng, (5) 1.23. Antibody: Streptavidin (p/n orb348765) at 1:40000 for 30 mins at RT. Block: BlockOut (p/n orb348644) for 30 mins at RT. Exposure: 1 sec.