Llama IgG1 isotype control Antibody

SKU: orb343832

Description

Llama IgG1 isotype control Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: SDS-PAGE
• Dilution Range: ELISA: User Optimized, FC: User Optimized, IF: User Optimized, WB: User Optimized
• Application Notes:
  Llama IgG1 isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow Cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. Llama IgG1 is buffered in 0.075 M Tris, 0.375 M NaCl, 25% glycerol.

Key Properties

• Isotype: IgG1
• Purity: Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain). No bands corresponding to llama IgG2 or IgG3 are observed.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide; Buffer: See application note.
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Llama IgG1 isotype, Llama IgG1 subclass isotype

SDS-PAGE of Llama IgG1 Isotype Control. Lane 1: Llama IgG1 (p/n orb343832), Non-reduced. Lane 2: Llama IgG2 (p/n orb343833), Non-reduced. Lane 3: Llama IgG3 (p/n orb343834), Non-reduced. M: 3 µl Opal Pre-stained Ladder. Lane 4: Llama IgG1, Reduced. Lane 5: Llama IgG2, Reduced. Lane 6: Llama IgG3, Reduced. Load: 1.0 µg per lane. Predicted/Observed size: Llama IgG1 Non-reduced 180 kDa; Reduced 55 kDa, 25 kDa.