Llama IgG1 isotype control Fluorescein Antibody

SKU: orb343835

Description

Llama IgG1 isotype control Fluorescein Antibody

Research Area

Protein Biochemistry

Images & Validation

• Tested Applications: FC, FLISA, IF, WB
• Dilution Range: FLISA: User Optimized, FC: User Optimized, IF: User Optimized, WB: User Optimized
• Application Notes:
  Llama IgG1 FITC Conjugated isotype control can be utilized as a control or standard reagent in Flow Cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting experiments where determination of sample isotype is important.

Key Properties

• Isotype: IgG1
• Purity: Llama IgG1 isotype control has been prepared from llama serum by multiple chromatography steps using a combination of protein A and protein G chromatography. Coomassie stained SDS-PAGE of non-reduced unconjugated llama IgG1 shows a band of ~150 kDa whereas the reduced form exhibits ~55 kDa (heavy chain) and ~25 kDa (light chain). No bands corresponding to unconjugated llama IgG2 or IgG3 are observed. Fluorescein Conjugated Llama IgG1 was proven by Dot Blot.
• Conjugation: FITC

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Llama IgG1 isotype, Llama IgG1 subclass isotype, Llama IgG1 Fluorescein Conjugated, Llama IgG1 FITC, Llama isotype control