LCN2 Antibody

SKU: orb1247309

Description

LCN2 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 22kDa band observed in Human Kidney lysates (calculated MW of 22.6kDa according to NP_005555.2). Recommended concentration: 0.01-0.03µg/mL.Immunohistochemistry:In paraffin embedded Human Spleen shows staining of cytoplasm in mostly binucleated cells, and of nucleoli in some of the non-dividing cells. Recommended concentration: 2-3µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-AILREDKDPQKMY
• Target: LCN2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LCN2, NGAL, lipocalin 2 (oncogene 24p3), HGNC:6526, uterocalin, oncogenic lipocalin 24p3, neutrophil gelatinase-associated lipocalin, lipocalin 2, MSFI, OTTHUMP00000022240, migration-stimulating factor inhibitor, neutrophil gelatinase-associated lipocalin, oncogene 24p3, siderocalin

orb1247309 (0.03 ug/ml) staining of Human Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247309 (2.5 ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

LCN2

UniProt

P80188

RefSeq (Protein):NP_005555.2