Lipocalin 2/LCN2 Antibody

SKU: orb312135

Description

Lipocalin 2/LCN2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  Western blot, 0.1-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat ELISA (cap) ,1-5μg/ml, Rat. Add 0.2ml of distilled water will yield a concentration of 500ug/ml

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived rat Lipocalin 2 recombinant protein (Position: Q21-N198). Rat Lipocalin 2 shares 64.4 % and 81.1 % amino acid (aa) sequence identity with human and mouse Lipocalin 2, respectively.
• Target: Neutrophil gelatinase-associated lipocalin
• Molecular Weight: 22 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Disclaimer: For research use only

Alternative Names

Neutrophil gelatinase-associated lipocalin; NGAL; Alpha-2-microglobulin-related protein; Alpha-2U globulin-related protein; Lipocalin-2; Siderocalin LCN2; p25; Lcn2

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of rat lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse lung tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in frozen section of mouse spleen tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in paraffin-embedded section of Mouse Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

IHC analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Lipocalin 2 was detected in paraffin-embedded section of Rat Spleen Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-Lipocalin 2 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Western blot analysis of Lipocalin 2 using anti-Lipocalin 2 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: Mouse Lung Tissue Lysate, Lane 2: Mouse Intestine Tissue Lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Lipocalin 2 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Lipocalin 2 at approximately 22 KD. The expected band size for Lipocalin 2 is at 22 KD.

Quick Database Links

Gene Symbol

Neutrophil gelatinase-associated lipocalin

UniProt

P30152