α-Linolenic acid

SKU: orb1303447

Description

α-Linolenic Acid (ALA) is an essential omega-3 fatty acid, sourced from seed oils, which must be obtained through diet. Research indicates its potential in cardiovascular and neurological studies, with applications in vitro and in vivo models for investigating lipid metabolism, cognitive function, and anti-thrombotic mechanisms.

Research Area

Signal Transduction

Key Properties

• CAS Number: 463-40-1
• MW: 278.43
• Purity: 99.81% (May vary between batches)
• Formula: C₁₈H₃₀O₂
• SMILES: CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O
• Target: PI3K,Akt
• Solubility: Ethanol:100 mg/mL (359.16 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:10 mg/mL (35.92 mM);DMSO:100 mg/mL (359.16 mM)

Bioactivity

• In Vivo:
  METHODS: To study the therapeutic effect on colitis in mice, α-Linolenic Acid (150-300 mg/kg, dispersed in 0.25% Tween-20) was administered by gavage to HFHSD-fed BABL/c mice once daily for nine weeks. At the end of nine weeks, experimental colitis was induced by intracolonic injection of TNBS. RESULTS: Low α-Linolenic Acid administration significantly ameliorated TNBS-induced clinical manifestations, body weight loss, spleen weight loss, and histologic damage. In contrast, high α-Linolenic Acid administration did not improve colitis and even exacerbated symptoms.HFHSD exacerbated TNBS-induced colitis via the Th1/Th17 pathway. Low α-Linolenic Acid showed a protective effect against TNBS-induced colitis via the Th1/Th2/Th17 pathway.
• In Vitro:
  METHODS: Osteosarcoma cells MG63, 143B and U2OS were treated with α-Linolenic Acid (10-200 µM) for 24 h. Cell viability was determined by MTT assay. RESULTS: α-Linolenic Acid showed a highly inhibitory effect on cell proliferation in a dose-dependent manner, with IC50 values of 51.69±0.14 µM in MG63 cells, 56.93±0.23 µM in 143B cells and 49.8±0.50 µM in U2OS cells. METHODS: Breast cancer cells MDA-MB-231 and MCF-7 were treated with α-Linolenic Acid (25-100 µM) for 24 h. Apoptosis was detected by flow cytometer. RESULTS: The percentage of apoptotic cells in the early (from 2.9% to 23.5% and from 5.5% to 16.7%, respectively) and late (from 0.6% to 3.4% and from 2.0% to 13.8%, respectively) apoptotic stages was significantly increased in MDA-MB-231 and MCF-7 cells treated with 100 µM α-Linolenic Acid. percentage significantly increased, which was significantly higher compared to untreated control cells.

Storage & Handling

• Storage: store at low temperature,store under nitrogen | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Seed, thrombotic, PI3K, αLinolenic acid, α-Linolenic acid, α Linolenic acid, oils, Linolenic acid, essential, anti-arrhythmic, a-Linolenic acid, Akt, Alpha-Linolenic Acid

Compound Identifiers

PubChem CID

5280934