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Catalog Number | orb1230432 |
---|---|
Category | Proteins |
Description | LIF Recombinant Protein |
Form/Appearance | Lyophilized |
Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses. |
Target | LIF |
UniProt ID | P15018 |
Protein Sequence | SPLPITPVNA TCAIRHPCHN NLMNQIRSQL AQLNGSANAL FILYYTAQGE PFPNNLDKLC GPNVTDFPPF HANGTEKAKL VELYRIVVYL GTSLGNITRD QKILNPSALS LHSKLNATAD ILRGLLSNVL CRLCSKYHVG HVDVTYGPDT SGKDVFQKKK LGCQLLGKYK QIIAVLAQAF |
Source | E. coli |
Biological Activity | Determined by its ability to stimulate the proliferation of human TF-1 cells. The expected ED50 is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. |
Storage | The recombinant protein is stable for at least 2 years from date of receipt at -20˚°C. Reconstituted protein is stable for at least 3 months when stored in working aliquots with a carrier protein at -20˚°C. As with any protein, exposing the recombinant protein to repeated freeze / thaw cycles is not recommended. When working with proteins care should be taken to keep recombinant protein at a cool and stable temperature. |
Alternative names | Leukemia Inhibitory Factor, Differentiation-stimul Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
> 98% as determined by SDS-PAGE and HPLC. | |
19.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
19.7 kDa | |
E.coli |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 40-55 kDa based on Tris-Bis PAGE result. |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC | |
Due to glycosylation, the protein migrates to 120-140 kDa based on Tris-Bis PAGE result. |