LIF Antibody

SKU: orb1248537

Description

LIF Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, WB
• Reactivity: Mouse, Rat
• Predicted Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 23kDa band observed in Mouse and Rat Colon lysates (calculated MW of 22.3kDa according to Mouse NP_032527.1. Primary incubation was 1 hour. Recommended concentration: 0.5-1.5µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: TPVNATCAIRHP
• Target: LIF
• Molecular Weight: Approx 23kDa
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LIF, leukemia inhibitory factor (cholinergic differentiation factor), CDF, DIA, HILDA, D factor, MLPLI, OTTHUMP00000198698, cholinergic differentiation factor, differentiation inhibitory activity, differentiation stimulating factor, differentiation-inducing factor, differentiation-stimulating factor, hepatocyte-stimulating factor III, human interleukin in DA cells, leukemia inhibitory factor, melanoma-derived LPL inhibitor

orb1248537 (0.5 ug/ml) staining of Mouse (A) and (1 ug/ml) Rat (B) Colon lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

LIF

UniProt

P15018

RefSeq (Protein):NP_002300.1