Lidocaine Hydrochloride hydrate

SKU: orb1224470

Description

Lidocaine, an amide local anesthetic, has anti-inflammatory properties in vitro and in vivo, possibly due to an attenuation of pro-inflammatory cytokines, intracellular adhesion molecule-1 (ICAM-1), and reduction of neutrophils influx.(In Vitro):Lidocaine (Lignocaine) (10 nM; 48 hours) decreases significantly cell proliferation.Lidocaine (1-10 nM; 24-72 hours) inhibits cell viability and achieves the most suppressing effects at the concentration of 10 nM and treatment time 48 hours.Lidocaine (10 nM; 48 hours) increases significantly the apoptotic cell rate.Lidocaine (10 nM; 48 hours) down-regulates Cyclin D1 and up-regulates p21 expression significantly.(In Vivo):Lidocaine (Lignocaine) causes completely reversible tail nerve block in rats. Mechanical nociception block produced by lidocaine has slower onset and faster recovery compared with thermal nociception block.

Research Area

Pharmacology & Drug Discovery

Key Properties

• CAS Number: 6108-05-0
• MW: 288.82
• Purity: >98% (HPLC)
• Formula: C₁₄H₂₂N₂O·HCl·H₂O
• SMILES: O.Cl.CCN(CC)CC(=O)NC1=C(C)C=CC=C1C
• Target: EGFR
• Solubility: DMSO: 10 mM

Bioactivity

• In Vivo:
  Lidocaine (Lignocaine) causes completely reversible tail nerve block in rats. Mechanical nociception block produced by lidocaine has slower onset and faster recovery compared with thermal nociception block.
• In Vitro:
  Lidocaine (Lignocaine) (10 nM; 48 hours) decreases significantly cell proliferation. Lidocaine (1-10 nM; 24-72 hours) inhibits cell viability and achieves the most suppressing effects at the concentration of 10 nM and treatment time 48 hours. Lidocaine (10 nM; 48 hours) increases significantly the apoptotic cell rate. Lidocaine (10 nM; 48 hours) down-regulates Cyclin D1 and up-regulates p21 expression significantly. Cell Proliferation Assay Cell line: The human gastric cancer cell line MKN45. Concentration: 10 nM. Incubation time: 48 hours. Result: Decreased significantly cell proliferation. Cell Viability Assay Cell line: The human gastric cancer cell line MKN45. Concentration: 1, 5 and 10 nM. Incubation time: 24, 48, 72 hours. Result: Inhibited MKN45 cell viability. Apoptosis Analysis Cell line: The human gastric cancer cell line MKN45. Concentration: 10 nM. Incubation time: 48 hours. Result: Increased significantly the apoptotic cell rate. Western blot analysis. Cell line: The human gastric cancer cell line MKN45. Concentration: 10 nM. Incubation time: 48 hours. Result: Down-regulated Cyclin D1 and up-regulated p21 expression significantly.

Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

EGFR