Levofloxacin hydrate

SKU: orb1308581

Description

Levofloxacin hydrate is the active L-isomer of ofloxacin, a third-generation fluorofluroquinolone antibiotic. It exerts its broad-spectrum antibacterial effect by inhibiting bacterial DNA gyrase, thereby disrupting DNA replication. This compound is widely used in research applications for studying bacterial infections and antibiotic mechanisms in both in vitro and in vivo models.

Research Area

Cell Biology, Infectious Disease & Virology, Molecular Biology

Key Properties

• CAS Number: 138199-71-0
• MW: 370.38
• Purity: 98.56% (May vary between batches)
• Formula: C₁₈H₂₀FN₃O₄·1/2H₂O
• SMILES: O.C[C@H]1COc2c(N3CCN(C)CC3)c(F)cc3c2n1cc(C(O)=O)c3=O.C[C@H]1COc2c(N3CCN(C)CC3)c(F)cc3c2n1cc(C(O)=O)c3=O
• Target: Topoisomerase,Virus Protease,DNA gyrase,Antibiotic,Antibacterial,DNA/RNA Synthesis
• Solubility: Ethanol:10 mg/mL (27 mM);H2O:5 mg/mL (13.5 mM);DMSO:50 mg/mL (135 mM);Methanol:10 mM

Bioactivity

• Cell Research:
  Cell suspension samples (50 mL) (2-5×104 mast cells in each tube for dispersed skin cells or 1 ×105 mast cells in each tube for rat peritoneal cells) in duplicate are equilibrated at 37℃ for 10 min, and then added to polypropylene tubes containing 450 mL test compounds dissolved in Tyrode solution. Histamine release reaction is allowed to proceed for a further 30 min. at the end of which time the reaction is terminated by adding 2 mL ice-cold Tyrode solution and the mixture is centrifuged (450 g for 5 min at 4℃). For determination of total histamine content, mast cells are disrupted in boiling water for 10 min. Histamine concentrations in the resulting supernatant and disrupted cells are determined by the enzyme immunoassay described above. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

airway inflammation, Antibiotic, Bacterial, BK Viremia, DNA/RNA Synthesis, DNA gyrase, DNAgyrase, Cravit hydrate, Cravit Hydrate, chronic periodontitis, Inhibitor, Levaquin hydrate, Levaquin Hydrate, Levofloxacin, Levofloxacin Hemihydrate, Levofloxacin Hydrate, Levofloxacin hydrate, Iquix Hydrate, Iquix hydrate, inhibit, Quixin Hydrate, Quixin hydrate, Topo IV, Topo II, Topoisomerase, Tavanic Hydrate, Tavanic hydrate

Compound Identifiers

PubChem CID

3033924