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Description
Research Area
Images & Validation
−| Tested Applications | FC, ICC, IF, IHC-Fr, IHC-P, IP, WB |
|---|---|
| Dilution Range | WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100 |
| Reactivity | Human, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide of human LEF1 |
| Target | LEF1 |
| Molecular Weight | Calculated MW: 44 kDa; Observed MW: 25-58 kDa |
| Purification | Affinity Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40%Glycerol, 0.01% sodium azide and 0.05% rAlbumin. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Immunocytochemistry analysis of LEF1 (green) in Jurkat using LEF1 antibody, and DAPI (blue).

Western blot analysis of LEF1 in C6 lysates using LEF1 antibody.

Western blot analysis of LEF1 in Jurkat lysates using LEF1 antibody.

Immunohistochemistry analysis of paraffin-embedded Human tonsil using LEF1 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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UniProt Details
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Protocol Information
LEF1 Rabbit pAb (orb1565541)
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