LDN-212320

SKU: orb1308133

Description

LDN-212320 (OSU-0212320) is a small molecule activator of the glutamate transporter EAAT2. It potently upregulates EAAT2 protein expression, increasing levels more than sixfold at concentrations below 5 μM within 24 hours. This compound is a valuable research tool for studying EAAT2 function in models of excitotoxicity, such as amyotrophic lateral sclerosis (ALS) and other neurological disorders, in both cellular and animal studies.

Research Area

Metabolism Research

Key Properties

• CAS Number: 894002-50-7
• MW: 293.39
• Purity: 98.18% (May vary between batches)
• Formula: C₁₇H₁₅N₃S
• SMILES: Cc1ccccc1CSc1ccc(nn1)-c1ccccn1
• Target: transporter
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.82 mM);DMSO:16.67 mg/mL (56.82 mM)

Bioactivity

• In Vivo:
  After LDN/OSU-0212320(a single i.p.; 40-mg/kg) treatment, EAAT2 protein levels and associated glutamate uptake increased by approximately 1.5- to 2-fold at 2 hours and by approximately 2- to 3-fold between 8 and 24 hours after injection. Even 72 hours after injection, an approximately 1.5-fold increase in EAAT2 protein levels could still be detected (data not shown). LDN/OSU-0212320–induced EAAT2 protein levels and glutamate uptake was dose-dependent .
• In Vitro:
  LDN/OSU-0212320 enhanced EAAT2 protein levels and glutamate uptake function but did not affect EAAT1 or EAAT3 protein levels and it also increased EAAT2 protein levels in a dose-dependent (EC50: 1.83 ± 0.27 μM) and time-dependent manner. LDN/OSU-0212320 treatment markedly prevented neuronal loss and degeneration, as assessed by MAP2 immunostaining .

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EAAT2, OSU 0212320, OSU0212320, OSU-0212320, Excitatory amino acid transporter 2, Glutamate transporter 1, GLT-1, LDN 212320, LDN212320, LDN-212320, LDN 0212320, LDN0212320, LDN-0212320, LDN/OSU 0212320, LDN/OSU0212320, LDN/OSU-0212320

Compound Identifiers

PubChem CID

7207425