L-Leucyl-L-Leucine methyl ester hydrochloride

SKU: orb1299549

Description

L-Leucyl-L-Leucine methyl ester hydrochloride is a cytotoxic dipeptide metabolite generated by immune cells. It is widely used in immunological research to selectively deplete cytotoxic lymphocytes and study lysosome-dependent cell death mechanisms in both in vitro and in vivo experimental models.

Research Area

Metabolism Research, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 6491-83-4
• MW: 294.81
• Purity: 99.63% (May vary between batches)
• Formula: C₁₃H₂₇ClN₂O₃
• SMILES: Cl.COC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CC(C)C
• Target: Endogenous Metabolite
• Solubility: DMSO:27.5 mg/mL (93.28 mM);10% DMSO+90% Saline:2.75 mg/mL (9.33 mM);5% DMSO+95% Saline:1.45 mg/mL (4.92 mM)

Bioactivity

• In Vivo:
  METHODS: To investigate the improvement of AD model and the mechanism, GW7647 (2.5 mg/kg) was administered to APP/PS1 transgenic mice daily for three months. RESULTS: GW 7647 reduced the Aβ burden and ameliorated cognitive deficits in APP/PS1 mice, and activation of PPAR-α by GW 7647 ameliorated the disruption of iron homeostasis and attenuated neuronal inflammation and lipid peroxidation in the brains of APP/PS1 mice, which may be related to the up-regulation of GPx4 transcripts mediated by the interaction between GPx4 non-coding regions and PPAR-α. METHODS: To investigate the effects on hepatocarcinogenesis in mice, GW 7647 (0.01% w/w) was administered dietary to wild-type, Para-null, or PPARA-humanized mice daily for 1, 5, and 26 weeks or chronically. RESULTS: In wild-type mice, GW 7647 caused high rates of hepatic expression of known PPARα target genes, hepatomegaly, hepatic MYC expression, hepatocytotoxicity, and hepatocellular carcinoma. In contrast, these effects were largely absent in Ppara-null mice and attenuated in PPARA-humanized mice, although hepatocarcinogenesis was observed in both genotypes.
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• In Vitro:
  METHODS: hSMCs cells were treated with GW 7647 (100-600 nM) for 24-96 h. DNA content was determined. RESULTS: GW 7647 treatment inhibited proliferation in a dose-dependent manner. METHODS: Gastric sinus mucosa was treated with GW 7647 (50 nM) for 10 min, and the expression levels of target proteins were detected by Western Blot. RESULTS: In gastric sinus mucosa, GW 7647 stimulated PI3K phosphorylation followed by Akt (Ser473) phosphorylation, which induced NOS1 phosphorylation.
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

pathway, lymphocytes, LLeucylLLeucine methyl ester hydrochloride, L-Leucyl-L-Leucine methyl ester Hydrochloride, LLOMe, L Leucyl L Leucine methyl ester hydrochloride, inhibit, monocytes, Inhibitor, Leu-Leu methyl ester, Leu-Leu-ome Hydrochloride, Leu-Leu-ome hydrochloride, leukocytes, Endogenous Metabolite, EndogenousMetabolite, endolysosomal, cytotoxic, polymorphonuclear, stress

Compound Identifiers

PubChem CID

15598024