L-BUTHIONINE-(SR)-SULFOXIMINE

SKU: orb1221012

Description

L-Buthionine-(SR)-sulfoximine is a cell-permeable and irreversible inhibitor of γ-glutamylcysteine synthetase (Ki <100 μM) that induces oxidative stress in cells by depleting GSH.

Key Properties

• CAS Number: 83730-53-4
• MW: 222.31
• Purity: >98% (HPLC)
• Formula: C₈H₁₈N₂O₃S
• SMILES: CCCCS(=N)(=O)CC[C@H](N)C(O)=O
• Target: γ-glutamylcysteine synthetase
• Solubility: H2O:41.67 mg/mL (187.44 mM; Need ultrasonic)

Bioactivity

• In Vivo:
  Animal model: C57BL/6J pun/pun mice. Dosage: 2 mM L-Buthionine-(S, R)-sulfoximine (BSO), 20 mM BSO, 2 mM BSO and 20 mM NAC, 20 mM NAC or unsupplemented water for 18 days from 0.5 to 18.5 d. p. c. The pH of supplemented water is as follows: 6.88, 20 mM BSO; 3.37, 2 mM BSO; 2.65, 2 mM BSO plus 20 mM NAC; and 2.58, 20 mM NAC. The pH of regular water used in our facility is ~4. Administration: Drinking water. Result: The average number of eye-spots (mean±SEM) is 5.36±0.29 (n = 46), 7.79±0.45 (n = 34) and 8.78±0.61 (n = 32) in untreated controls, 2 mM L-Buthionine-(S, R)-sulfoximine (BSO) and 20 mM BSO treated mice, respectively. The 2 mM BSO treatment results in ~30% more eye-spots, and the 20 mM treatment results in 40% more eye-spots compared with untreated mice.
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Storage & Handling

• Storage: Storage temperature: -20°C. Stability: ≥ 2 years
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

L-Butionine sulfoximine

Quick Database Links

Gene Symbol

γ-glutamylcysteine synthetase