Cart summary

You have no items in your shopping cart.

KRT8 Antibody / Cytokeratin 8

Catalog Number: orb248434

DispatchUsually dispatched within 5-10 working days
$ 410.00
Catalog Numberorb248434
CategoryAntibodies
DescriptionCytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). Cytokeratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibody that recognizes only cytokeratin 8 and 18. Cytokeratin 8 exists on several types of normal and neoplastic epithelia, including many ductal and glandular epithelia such as colon, stomach, small intestine, trachea, and esophagus as well as in transitional epithelium. antibody to Cytokeratin 8 does not react with skeletal muscle or nerve cells. Epithelioid sarcoma, chordoma, and adamantinoma show strong positivity corresponding to that of simple epithelia (with antibodies against Cytokeratin 8, 18 and 19). Reportedly, Cytokeratin 8 antibody is useful for the differentiation of lobular (“ring-like, perinuclear”) from ductal (“peripheral-predominant”) carcinoma of the breast.
Species/HostMouse
ClonalityMonoclonal
Clone NumberH1
Tested applicationsFACS, IF, IHC-P, WB
ReactivityHuman, Rat
IsotypeMouse IgG1, kappa
ImmunogenA cytoskeleton preparation containing Cytokeratin 8 was used as the immunogen for this antibody.
Antibody TypePrimary Antibody
Dilution rangeWestern blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
PurityProtein G affinity chromatography
ConjugationUnconjugated
Formula0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Hazard InformationThis Cytokeratin 8 antibody is available for research use only.
Entrez3856
StorageStore the Cytokeratin 8 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
NoteFor research use only
Application notesThe concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Expiration Date12 months from date of receipt.
KRT8 Antibody / Cytokeratin 8

Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 8 antibody (clone H1); Red = isotype control, Blue = Cytokeratin 8 antibody.

KRT8 Antibody / Cytokeratin 8

Western blot testing of human HCT-116 lysate with Cytokeratin 8 antibody.

KRT8 Antibody / Cytokeratin 8

Western blot testing of A431 lysate with Cytokeratin 8 antibody

KRT8 Antibody / Cytokeratin 8

Immunofluorescent staining of permeabilized human MCF7 cells with Cytokeratin 8 antibody (clone H1, green) and Reddot nuclear stain (red).

KRT8 Antibody / Cytokeratin 8

Immunofluorescent staining of permeabilized human HCT-116 cells with Cytokeratin 8 antibody (clone H1, green) and Reddot nuclear stain (red).

KRT8 Antibody / Cytokeratin 8

IHC testing of human colon carcinoma stained with Cytokeratin 8 antibody

  • KRT8 Antibody / Cytokeratin 8 [orb2637802]

    FACS,  IF,  IHC-P,  WB

    Human, Rat

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • KRT8 Antibody / Cytokeratin 8 [orb639772]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg
  • KRT8 Antibody / Cytokeratin 8 [orb2643484]

    IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    100 μg
  • KRT8 Antibody / Cytokeratin 8 [orb1151726]

    IHC-P,  WB

    Human

    Mouse

    Recombinant

    Unconjugated

    100 μg
  • KRT8 Antibody / Cytokeratin 8 [orb749629]

    FACS,  IF,  IHC-P,  WB

    Human

    Mouse

    Monoclonal

    Unconjugated

    20 μg