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Catalog Number | orb1471856 |
---|---|
Category | Proteins |
Description | KLRF1 Human Recombinant produced in HEK293 Cells is a single, glycosylated polypeptide chain containing 414 amino acids (60-231a.a) and having a molecular mass of 47.1kDa.KLRF1 is fused to a 242 amino acid hIgG-His-Tag at C-terminus & purified by proprietary chromatographic techniques. |
Form/Appearance | Sterile filtered colorless solution. |
Purity | Greater than 85.0% as determined by SDS-PAGE. |
Protein Sequence | DGSLLVSQGV LLKCQKGSCS NATQYEDTGD LKVNNGTRRN ISNKDLCASR SADQTVLCQS EWLKYQGKCY WFSNEMKSWS DSYVYCLERK SHLLIIHDQL EMAFIQKNLR QLNYVWIGLN FTSLKMTWTW VDGSPIDSKI FFIKGPAKEN SCAAIKESKI FSETCSSVFK WICQYLEPKS CDRTHTCPPC PAPELLGGPS VFLFPPKPKD TLMISRTPEV TCVVVDVSHE DPEVKFNWYV DGVEVHNAKT KPREEQYNST YRVVSVLTVL HQDWLNGKEY KCKVSNKALP APIEKTISKA KGQPREPQVY TLPPSRDELT KNQVSLTCLV KGFYPSDIAV EWESNGQPEN NYKTTPPVLD SDGSFFLYSK LTVDKSRWQQ GNVFSCSVMH EALHNHYTQK SLSLSPGKHH HHHH |
Source | HEK293 Cells |
Biological Activity | Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human KLRF1 coated plates 2 ug/ml. This effect is more to 60%. |
Storage | Stability: Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles |
Buffer/Preservatives | The KLRF1 solution (0.25mg/1ml) contains phosphate buffered saline (pH7.4) and 10% glycerol. |
Alternative names | NKp80, KLRF1, C-type lectin domain family 5 member Read more... |
Note | For research use only |
Application notes | Recombinant & Natural Proteins |
Expiration Date | 6 months from date of receipt. |
98.00% | |
25-35 KDa (reducing condition) |
98.00% | |
45.37 kDa (predicted). Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. |
45.79 kDa (predicted); 52.5 kDa (reducing contition, due to glycosylation) |
98.00% | |
47.8 kDa (predicted); 59 kDa (reducing conditions) |
> 90%, determined by SDS-PAGE | |
This protein contains the cynomolgus KLRF1(Q8MI05) (Val66-Tyr231) was expressed with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |