Ketorolac tromethamine salt

SKU: orb1309385

Description

Ketorolac tromethamine is a non-selective cyclooxygenase (COX-1 and COX-2) inhibitor with analgesic, anti-inflammatory, and antipyretic properties. It is widely used in research to investigate pain mechanisms and inflammation in both in vitro enzymatic assays and in vivo animal models.

Research Area

Immunology & Inflammation, Neuroscience, Pharmacology & Drug Discovery

Key Properties

• CAS Number: 74103-07-4
• MW: 376.4037
• Purity: 99.85% (May vary between batches)
• Formula: C₁₉H₂₄N₂O₆
• SMILES: NC(CO)(CO)CO.OC(=O)C1CCN2C1=CC=C2C(=O)C1=CC=CC=C1
• Target: COX
• Solubility: DMSO:65 mg/mL (172.69 mM);Ethanol:70 mg/mL (185.97 mM);H2O:14 mg/mL (37.19 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.31 mM)

Bioactivity

• Target IC50:
  COX-1:20 nM|COX-2:20 nM
• In Vivo:
  Ketorolac inhibits prostaglandin synthesis in HEL cells (COX-1) (IC50=0.025 μM) and LPS-stimulated Mono Mac 6 cells (COX-2) (IC50=0.039 μM). In human osteoblasts (hOB), Ketorolac dose-dependently suppresses thymidine incorporation and proliferation at the G0/G1 phase, blocking cell cycle progression.
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• In Vitro:
  In models of acetic acid-induced writhing (ID50=0.24 mg/kg), carrageenan-induced hyperalgesia in the paw (ID50=0.29 mg/kg), and carrageenan-induced rat paw edema (ID50=0.08 mg/kg), (R,S)-Ketorolac has demonstrated efficacy.
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• Cell Research:
  Human osteoblasts cells are exposed to Ketorolac for 24 hours. Thymidine incorporation is assessed by the TopCount Microplate Scintillation and Luminescence Counters through adding [3H]-thymidine to cultures 4 hours prior to harvesting. Cell cycle distribution is determined by using propidium iodide in flow cytometer, and cell apoptosis or necrosis is detected using the Annexin V-FITC Apoptosis Detection Kit. (Only for Reference)
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Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Ketorolac, Ketorolac tris, Ketorolac tris salt, Ketorolac tromethamine, Ketorolac tromethamine salt, Acular, Acular LS, COX-1, COX-2, COX, Toradol, RS 37619, RS37619, RS-37619

Compound Identifiers

PubChem CID

84003