KC7F2

SKU: orb1304466

Description

KC7F2 is a small molecule inhibitor of the HIF-1 signaling pathway, exhibiting promising anti-tumor properties. It is utilized in cancer research to study hypoxia response mechanisms, demonstrating efficacy in both cellular assays and in vivo tumor models.

Research Area

Cell Biology, Neuroscience

Key Properties

• CAS Number: 927822-86-4
• MW: 570.4
• Purity: >98%
• Formula: C₁₆H₁₆Cl₄N₂O₄S₄
• SMILES: NS(=O)(=O)c1cc(Cl)cc(CCSSCCc2cc(Cl)cc(c2Cl)S(N)(=O)=O)c1Cl
• Target: Transcription factor
• Solubility: Soluble in DMSO (up to at least 25 mg/ml)

Bioactivity

• Target IC50:
  HIF1α:20 μM
• In Vivo:
  KC7F2 significantly reduces the latent period in the pentylenetetrazole kindling rat model and increases the rate of spontaneous recurrent seizures during the chronic stage in the lithium-pilocarpine rat model.
• In Vitro:
  KC7F2 inhibits HRE-driven transcription and decreases HIF-1α protein levels in LN229-HRE-AP cells. KC7F2 shows a dose-response cytotoxicity with IC50 of approximately 15 to 25 μM in cancer cells MCF7, LNZ308, A549, U251 mg, and LN229. In D54 mg glioma cells, KC7F2 inhibits colony formation, especially under hypoxia. In hypoxic microglial cultures, KC7F2 downregulates the expression of TfR and DMT, and reduces the HIF-1α mediated iron accumulation.
• Cell Research:
  Cells are seeded onto 96-well plates (4 × 103/well) and cultured under normoxic (21% O2) and hypoxic (1% O2) conditions with different concentrations of KC7F2 for 72 h or treated for various times with 20 μM KC7F2. For proliferation analysis, cells are fixed with 50% trichloroacetic acid for 1 h at 4°C, followed by staining with 0.4% sulforhodamine B dissolved in 1% acetic acid for 30 min at room temperature. Plates are washed five times with 1% acetic acid to remove unbound dye. Bound dye is dissolved by adding 10 mM unbuffered Tris base. Cell proliferation is calculated by measuring OD values at 564 nm using a spectrophotometer.(Only for Reference)

Storage & Handling

• Storage: -20°C
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

inhibit, KC7F2, KC-7F2, Inhibitor, Hypoxia-inducible factors, HIF, HIF Prolyl-Hydroxylase, HIF ProlylHydroxylase, HIF/HIF Prolyl-Hydroxylase, HIF/HIFProlylHydroxylase, HIF/HIF ProlylHydroxylase, HIFs, HIFProlylHydroxylase, HIF-PH, HIF-1α

Compound Identifiers

PubChem CID

91895227