JW 55

SKU: orb1307402

Description

JW 55

Research Area

Epigenetics & Chromatin, Molecular Biology, Signal Transduction, Stem Cell & Developmental Biology

Key Properties

• CAS Number: 664993-53-7
• MW: 434.48
• Purity: 99.76%
• Formula: C₂₅H₂₆N₂O₅
• SMILES: COc1ccc(cc1)C1(CNC(=O)c2ccc(NC(=O)c3ccco3)cc2)CCOCC1
• Target: PARP,Wnt/beta-catenin
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (4.6 mM);DMSO:50 mg/mL (115.08 mM);Ethanol:100 mM

Bioactivity

• Target IC50:
  TNKS2 (auto-PARsylation):830 nM|TNKS1 (auto-PARsylation):1.9 μM
• In Vivo:
  JW 55 (100 mg/kg, orally) reduces tumor development in conditional Apc knockout mice. JW55 reduces XWnt8-induced axis duplication inXenopus embryos and Tamoxifen-induced polyposis formation in conditional APC mutant mice.
• In Vitro:
  JW 55 (JW55) is a potent and selective inhibitor of the canonical Wnt pathway. Wnt3a-induced HEK293 cells containing a transiently transfected ST-Luc (SuperTop-luciferase) reporter show inhibition by JW55 with an IC50 value of 470 nM. JW55 is effective in the range of 1 to 5 μM in SW480 cells and 0.01 to 5 μM in HCT-15 cells. JW55 is effective in the range of 1 to 5 μM in SW480 cells and 0.01 to 5 μM in HCT-15 cells.
• Cell Research:
  JW 55 (JW55) is prepared in DMSO and stored, and then diluted with appropriate medium. A total of 1,000 SW480 or RKO cells are seeded in 96-well plates. The day after, the cell culture medium is exchanged to solutions that contained 0.1% DMSO or 10 μM JW55 for RKO cells and 0.1% DMSO or 10, 5, or 1 μM JW55 for SW480 cells. All samples consist of a minimum of 6 replicates. The plate is incubated in an IncuCyte inside a cell culture incubator. Images are captured every second hour to monitor proliferation.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

PARP, JW 55, JW55, JW-55, inhibit, Inhibitor, betacatenin, beta-catenin, bcatenin, poly ADP ribose polymerase, Wnt/β-catenin, Wnt/betacatenin, Wnt/b-catenin, Wnt, TNKS1, TNKS2, βcatenin

Compound Identifiers

PubChem CID

2946601