JR-AB2-011

SKU: orb1309521

Description

JR-AB2-011 is a potent and selective mTORC2 inhibitor (IC₅₀ = 0.36 µM) that acts by disrupting the Rictor-mTOR interaction (Kᵢ = 0.19 µM). It demonstrates cytotoxic effects in glioblastoma cell models, making it a valuable research tool for studying mTORC2 signaling in cancer biology, both in vitro and in vivo.

Research Area

Signal Transduction

Key Properties

• CAS Number: 2411853-34-2
• MW: 398.28
• Purity: 99.41% (May vary between batches)
• Formula: C₁₇H₁₄Cl₂FN₃OS
• SMILES: CC1CN(C(=O)Nc2ccc(Cl)c(Cl)c2)\C(S1)=N\c1ccc(F)cc1
• Target: mTOR
• Solubility: DMSO:61.5 mg/mL (154.41 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (5.02 mM)

Bioactivity

• Target IC50:
  mTORC2:0.36 μM
• In Vivo:
  Mice administered JR-AB2-011 through intraperitoneal injection at doses of 4 mg/kg or 20 mg/kg daily for 10 days exhibited significant suppression of tumor growth compared to those treated with a vehicle control. Specifically, the lower dose achieved a 74% reduction in tumor growth rate with a 10-day delay in tumor progression, while the higher dose resulted in an 80% reduction and a 12-day delay. This study utilized female C.B.-17-scid mice implanted with LN229 cells, demonstrating that JR-AB2-011 is potent in inhibiting tumor growth at both tested concentrations.
• In Vitro:
  JR-AB2-011 (1 μM; 24 hours) shows good anti-GBM properties, blocks mTORC2 signaling and Rictor association with mTOR . JR-AB2-011 (0.5-2 μM; 48 hours) displays the least toxicity to normal neurons with no significant cytotoxic effects for concentrations up to 10 mM compared to CID613034 . Apoptosis Analysis Cell Line: U87 GBM cells; LN229 GBM cells Concentration: 1 μM Incubation Time: 24 hours Result: Had good anti-GBM properties and blocked mTORC2 signaling and Rictor association with mTOR. Cell Cytotoxicity Assay Cell Line: Normal mature human neurons Concentration: 0.5, 1, 2 μM Incubation Time: 48 hours Result: Displayed the least toxicity to normal neurons with no significant cytotoxic effects for concentrations up to 10 mM.

Storage & Handling

• Storage: keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

JR AB2 011, JRAB2011, JR-AB-2-011, JR-AB2-011, mTORC2

Compound Identifiers

PubChem CID

138319699