JIB-04

SKU: orb1307293

Description

JIB-04 (NSC 693627) is a small molecule inhibitor that broadly targets the Jumonji family of histone demethylases. It is utilized in epigenetic research to probe the role of histone methylation in both cellular studies and animal models of cancer and other diseases.

Research Area

Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 199596-05-9
• MW: 308.76
• Purity: 99.68% (May vary between batches)
• Formula: C₁₇H₁₃ClN₄
• SMILES: Clc1ccc(N\N=C(/c2ccccc2)c2ccccn2)nc1
• Target: Histone Demethylase,Histone Methyltransferase,Apoptosis
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.48 mM);DMSO:36.43 mg/mL (117.99 mM)

Bioactivity

• Target IC50:
  JMJD2D:290 nM|JMJD2E:340 nM|JMJD2A:445 nM|JMJD2B:435 nM|JARID1A:230 nM
• In Vivo:
  In lung and prostate cancer cell lines (IC50=10 nM), JIB-04 downregulates proliferation genes and inhibits cell growth.
• In Vitro:
  In the H358 and A549 mouse xenograft models, JIB-04 inhibits the activity of Jumonji domain-containing histone demethylases, thereby suppressing tumor cell growth.
• Cell Research:
  For cell viability assays, cells are plated at 1500-3000 cells/well in 96 well plates and treated the next day with increasing doses of compound over 4 days and their viability assessed by standard MTS assays using Promega’s Cell Titer or Cell Titer-Glo reagents according to the manufacturer’s protocols. Absorbance at 490 nm and 650 nm or luminescence is measured by a Spectra Max or a FlouroStar Omega plate reader. Data are normalized to the untreated controls (100% viability). Each cell line is tested in 2-5 independent assays, each containing 4-8 replicates. IC50 values are calculated using DIVISA, a high-throughput software, developed in hous, for storing and analyzing drug sensitivity assays. Dose-response curves are plotted using a non-linear regression model and IC50s are determined from the fitted curves. The average IC50 derived from 2-5 independent assays, each containing 4-8 replicates is reported. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Histone Demethylase, HistoneDemethylase, Histone Methyltransferase, HistoneMethyltransferase, Inhibitor, JARID1A, JIB 04, JIB04, JIB-04, JMJD2D, JMJD2E, JMJD2A, JMJD2B, NSC 693627, inhibit, NSC693627, NSC-693627

Compound Identifiers

PubChem CID

6519698