JIB-04

SKU: orb1307293

Description

JIB-04

Research Area

Cell Biology, Epigenetics & Chromatin

Key Properties

• CAS Number: 199596-05-9
• MW: 308.76
• Purity: 98.44%
• Formula: C₁₇H₁₃ClN₄
• SMILES: Clc1ccc(N\N=C(/c2ccccc2)c2ccccn2)nc1
• Target: Histone Methyltransferase,Apoptosis,Histone Demethylase
• Solubility: 10% DMSO+40% PEG300+5% Tween 80+45% Saline:2 mg/mL (6.48 mM);DMSO:36.43 mg/mL (117.99 mM)

Bioactivity

• Target IC50:
  JMJD2D:290 nM|JMJD2E:340 nM|JMJD2A:445 nM|JMJD2B:435 nM|JARID1A:230 nM
• In Vivo:
  In lung and prostate cancer cell lines (IC50=10 nM), JIB-04 downregulates proliferation genes and inhibits cell growth.
• In Vitro:
  In the H358 and A549 mouse xenograft models, JIB-04 inhibits the activity of Jumonji domain-containing histone demethylases, thereby suppressing tumor cell growth.
• Cell Research:
  For cell viability assays, cells are plated at 1500-3000 cells/well in 96 well plates and treated the next day with increasing doses of compound over 4 days and their viability assessed by standard MTS assays using Promega’s Cell Titer or Cell Titer-Glo reagents according to the manufacturer’s protocols. Absorbance at 490 nm and 650 nm or luminescence is measured by a Spectra Max or a FlouroStar Omega plate reader. Data are normalized to the untreated controls (100% viability). Each cell line is tested in 2-5 independent assays, each containing 4-8 replicates. IC50 values are calculated using DIVISA, a high-throughput software, developed in hous, for storing and analyzing drug sensitivity assays. Dose-response curves are plotted using a non-linear regression model and IC50s are determined from the fitted curves. The average IC50 derived from 2-5 independent assays, each containing 4-8 replicates is reported. (Only for Reference)

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Apoptosis, Histone Demethylase, HistoneDemethylase, Histone Methyltransferase, HistoneMethyltransferase, Inhibitor, JARID1A, JIB 04, JIB04, JIB-04, JMJD2D, JMJD2E, JMJD2A, JMJD2B, NSC 693627, inhibit, NSC693627, NSC-693627

Compound Identifiers

PubChem CID

6519698