JBS Deep Purple

SKU: orb65678

Description

Crystallization screening with high concentrations of precipitant and salt may lead to the formation of salt crystals. It is quite difficult to make a distinction between these false positives and true protein crystals. Staining of crystals with appropriate dyes is a very straightfor-ward method to differentiate between macromolecular crystals and salt crystals. Protein and salt crystals differ substantially in their solvent content. Small crystal dyes, like Biorbyt Deep Purple, are able to permeate the solvent channels of a protein, thus coloring the protein purple. In contrast, salt crystals are tightly packed and do not possess large solvent channels. They will therefore remain colourless.

Images & Validation

• Application Notes:
  Biorbyt Deep Purple is a crystal dye used to stain macromolecular crystals, i.e. protein, peptide and nucleic acid crystals in order to differentiate them from small molecules and salt crystals.

Key Properties

• CAS Number: 548-62-9
• MW: Theoretical MW: 407.98 g/mol
• Formula: C₂₅H₃₀N₃Cl
• SMILES: [Cl-].N(c1ccc(C(=C2C=CC(=[N+](C)C)C=C2)c2ccc(N(C)C)cc2)cc1)(C)C

Storage & Handling

• Storage: store at ambient temperature
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Crystal Violet

Compound Identifiers

PubChem CID

11057