izTRAIL, Soluble Recombinant Protein

• Catalog Number: orb1230153
• Category: Proteins
• Description: izTRAIL, Soluble Recombinant Protein
• Target: TNFSF10
• Tag: isoleucine zipper motif Tag
• Form/Appearance: Lyophilized
• Concentration: 0.1mg/ml after reconstitution.
• Buffer/Preservatives: Contains 20mM TRIS-Cl, 0.5M arginine-HCl, 100mM NaCl, 0.02% Tween 20. Reconstitute with 100 μl sterile water.
• Purity: >95% (SDS-PAGE). Endotoxin level is less than 0.01EU/ μg purified protein (LAL test; Lonza).
• Protein Sequence: The extracellular domain of human TRAIL (aa 95-281) is fused at the N-terminus to an isoleucine zipper motif.
• UniProt ID: Q02223
• Application notes: This recombinant proteins is for research use only.
• Source: E. coli
• Biological Activity: Induces apoptosis in vitro in different human cancer cell lines with an EC50 of 5-200ng/ml, depending on the individual cell line used. Recombinant izTRAIL does not kill 4-day cultures of primary human hepatocytes (PHH) at concentrations of at least up to 1ug/ml (Ganten 2006). Good bioavailability in vivo, shows no toxic effects in mice at doses of at least up to 500ug per day (Wissink 2006).
• Storage: Stable for at least 6 months after receipt when stored at -20˚°C.
• Alternative names: Apo-2L, TNFSF10, CD253
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only
• NCBI: P50591
• Expiration Date: 6 months from date of receipt.