IRF1-IN-1

SKU: orb2814595

Description

IRF1-IN-1 is a small molecule inhibitor of IRF1 that blocks its recruitment to the Caspase 1 promoter, thereby suppressing the cleavage of downstream targets like GSDMD, IL-1β, and PARP1. This mechanism has shown protective effects against skin inflammation in relevant research models, making it a useful tool for studying pyroptosis and inflammatory dermatoses in vitro and in vivo.

Research Area

Cell Biology, Epigenetics & Chromatin, Immunology & Inflammation, Molecular Biology, Protein Biochemistry

Key Properties

• CAS Number: 701225-07-2
• MW: 440.52
• Purity: 99.88%
• Formula: C₂₂H₂₄N₄O₄S
• SMILES: O=C1C=2C=CC=CC2N=CN1CCC(=O)NC3=CC=C(C=C3)S(=O)(=O)N4CCCCC4
• Target: Interleukin,Pyroptosis,PARP,IFNAR,IL Receptor,Caspase
• Solubility: DMSO:80 mg/mL (181.6 mM);10% DMSO+40% PEG300+5% Tween-80+45% Saline:5 mg/mL (11.35 mM)

Bioactivity

• In Vivo:
  Method: A radiogenic skin injury mouse model was established using whole-body irradiation with a 6-MeV electron beam at a dose of 35 Gy and a dose rate of 1000 cGy/min. Mice were anesthetized prior to irradiation with an intraperitoneal injection of pentobarbital sodium (1%, 30 mg/kg). IRF1-IN-1 was administered subcutaneously at a dose of 100 μg/day, once every other day as a pretreatment before irradiation. Result: IRF1-IN-1 significantly reduced acute skin inflammatory manifestations such as erythema and exudation, and accelerated the healing process. It also exhibited protective effects on the function and structural integrity of radiation-induced lesions in the claws.
• In Vitro:
  Method: Various cell models (HELF, HaCaT, WS1, and K150) were treated with IRF1-IN-1 at concentrations ranging from 20 to 50 μM. The effects were evaluated under conditions such as irradiation (20 Gy), NSP-10 plasmid transfection, or SARS-CoV-2 pseudovirus infection to assess IRF1 activity, cell death-related signaling, and mitochondrial function. Result: IRF1-IN-1 inhibited IRF1-mediated transcriptional regulation of downstream genes (e.g., CASP1), reduced IRF1 activation induced by NSP-10 or viral infection, alleviated radiation-induced cell death, maintained mitochondrial activity and ROS levels, and significantly suppressed the cleavage of Caspase 1, GSDMD, IL-1, and PARP1, demonstrating a strong cytoprotective effect.

Storage & Handling

• Storage: Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Compound Identifiers

PubChem CID

1291315