IR-820

SKU: orb1819335

Description

IR-820 (New Indocyanine Green) is a near-infrared fluorescent dye with superior stability and tissue penetration, exhibiting excitation/emission maxima at 710/820 nm. It serves as an effective blood pool contrast agent for non-invasive in vivo imaging, enabling the detection and quantification of pathological tissues in preclinical animal models.

Key Properties

• CAS Number: 172616-80-7
• MW: 849.47
• Purity: 98.64%
• Formula: C₄₆H₅₀ClN₂NaO₆S₂
• SMILES: [Na+].CC1(C)C(=CC=C2CCCC(C=CC3=[N+](CCCCS([O-])(=O)=O)c4ccc5ccccc5c4C3(C)C)=C2Cl)N(CCCCS([O-])(=O)=O)c2ccc3ccccc3c12
• Target: Others
• Solubility: DMSO:4.5 mg/mL (5.3 mM)

Bioactivity

• In Vitro:
  Instructions: I. Solution preparation 1. Stock solution: Prepare 1–10 mM stock solution, dissolve in sterile water or DMSO, and adjust the concentration according to experimental needs. 2. Working concentration: Dilute the stock solution to 10–50 µM (in vitro) or 0.5–1 mg/kg (in vivo) according to the experimental objectives. Notes: Powder: It is recommended to store in a dry environment away from light, and the temperature is controlled at -20°C or lower. Solution: It can be stored at 4°C for short-term storage, and it needs to be aliquoted and kept away from light for long-term storage, and stored at -20°C to avoid repeated freezing and thawing. II. Bioimaging experiment IR820 is commonly used in near-infrared fluorescence imaging (NIRF) and is suitable for in vivo or in vitro imaging studies. Experimental steps: In vitro experiment: Directly add IR820 working solution to the cell culture system and incubate for 30–60 minutes. In vivo experiment: Dilute IR820 and inject it into the experimental animal through the tail vein. The signal is detected by near-infrared imaging equipment during imaging. III. Photothermal/photodynamic therapy IR820 has photothermal and photosensitivity properties and can be used for photothermal therapy or photodynamic therapy of tumors. Illumination conditions: Use 808 nm laser irradiation, and the light intensity and time are optimized according to the experiment (such as 1–2 W/cm², 5–10 minutes). Concentration reference: Dilute IR820 to 5–20 µM (in vitro) or 0.5–2 mg/kg (in vivo) according to the treatment goal. IV. Fluorescence detection parameters Excitation wavelength: about 700–800 nm Emission wavelength: about 820–850 nm Precautions Light-avoiding operation: IR820 is sensitive to light, and strong light should be avoided during the operation. Fresh preparation: It is recommended to prepare it before use to prevent degradation of the dissolving solution. Control: A blank group and a control group should be set up in the experiment to ensure reliable data.

Storage & Handling

• Storage: keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice/Shipping at ambient temperature.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

New Indocyanine Green, IR 820, IR820, IR-820, Dye reagent

Compound Identifiers

PubChem CID

57370533